Antioxidant capacity and concentration of redox-active trace mineral in fully weaned intra-uterine growth retardation piglets.
Antioxidant capacity and concentration of redox-active trace mineral in fully weaned intra-uterine growth retardation piglets.
复制标题
完全断奶子宫内生长迟缓仔猪的抗氧化能力和氧化还原活性微量矿物质的浓度。
DOI：
10.1186/s40104-015-0047-7
复制
发表时间：
2015
影响因子：
7
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Zhang H;Li Y;Wang T
宫内生长迟缓（IUGR）仔猪断奶后氧化还原状态的研究很少。对24头母猪的新生仔猪进行称重，在21 d断奶，并饲喂起始饲料直至取样。在断奶后14天进行取样。当仔猪的出生体重低于总群体的平均出生体重2 SD时，将仔猪定义为IUGR。在断奶时，从每个类别（即IUGR或正常出生体重（NBW）仔猪）中选择体重几乎相等的18头仔猪，然后分配至两个处理组，每组6头，每个围栏3头仔猪。与正常体重组相比，IUGR组仔猪断奶后前2周的平均日增重、平均日采食量和饲料转化率均极显著降低（P <0.001），差异显著（P = 0.003）。IUGR组血浆总抗氧化能力（P = 0.019）、总超氧化物歧化酶（T-SOD，P = 0.023）和血浆铜蓝蛋白（P = 0.044）降低，丙二醛（P = 0.040）和蛋白质羰基（P = 0.010）升高。IUGR仔猪肝脏中T-SOD（P = 0.005）、Cu/Zn-SOD（P = 0.002）和过氧化氢酶（P = 0.049）活性均低于NBW仔猪。IUGR组仔猪单位Cu/Zn-SOD蛋白酶活性显著低于NBW组（P = 0.023）。此外，IUGR仔猪血浆（P = 0.001）和肝脏（P = 0.014）中铜的蓄积量以及肝脏中铁（P = 0.002）和锌（P = 0.048）的含量均降低。与NBW相比，IUGR下调了仔猪肝脏Cu/Zn-SOD mRNA的表达（P = 0.021）。结果表明，IUGR降低了断奶仔猪的抗氧化能力，导致氧化损伤，这可能与氧化还原活性的微量元素水平下降有关。这项研究强调了氧化还原状态在IUGR后代的重要性，并提供了一个合理的饮食干预，旨在补充微量矿物质，以减轻氧化损伤，并在未来恢复氧化还原平衡。
The redox status of intra-uterine growth retardation (IUGR) piglets post-weaning has been poorly studied. Newborns from twenty-four sows were weighted, weaned at 21 d and fed a starter diet until sampling. Sampling was done at 14 d post-weaning. A piglet was defined as IUGR when its birth weight was 2 SD below the mean birth weight of the total population. At weaning, eighteen piglets with nearly equal body weight from each category (i.e. IUGR or normal birth weight (NBW) piglets) were selected and then allocated to two treatments, consisted of six replicates with each pen having three piglets. Compared with NBW group, IUGR significantly decreased average daily gain (P < 0.001), average daily feed intake (P = 0.003), and feed efficiency (P < 0.001) of piglets during the first two weeks post-weaning. IUGR decreased the activities of total antioxidant capacity (P = 0.019), total superoxide dismutase (T-SOD, P = 0.023), and ceruloplasmin (P = 0.044) but increased the levels of malondialdehyde (P = 0.040) and protein carbonyl (P = 0.010) in plasma. Similarly, the decreased activities of T-SOD (P = 0.005), copper- and zinc-containing superoxide dismutase (Cu/Zn-SOD, P = 0.002), and catalase (P = 0.049) was observed in the liver of IUGR piglets than these of NBW piglets. IUGR decreased hepatic Cu/Zn-SOD activity (P = 0.023) per unit of Cu/Zn-SOD protein in piglets when compared with NBW piglets. In addition, IUGR piglets exhibited the decreases in accumulation of copper in both plasma (P = 0.001) and liver (P = 0.014), as well as the concentrations of iron (P = 0.002) and zinc (P = 0.048) in liver. Compared with NBW, IUGR down-regulated mRNA expression of Cu/Zn-SOD (P = 0.021) in the liver of piglets. The results indicated that IUGR impaired antioxidant capacity and resulted in oxidative damage in fully weaned piglets, which might be associated with the decreased levels of redox-active trace minerals. This study highlights the importance of redox status in IUGR offspring and provides a rationale for alleviating oxidative damage by dietary interventions aiming to supplement trace minerals and to restore redox balance in the future.