GENES REGULATING HLA CLASS-I ANTIGEN EXPRESSION IN T-B LYMPHOBLAST HYBRIDS
GENES REGULATING HLA CLASS-I ANTIGEN EXPRESSION IN T-B LYMPHOBLAST HYBRIDS
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DOI：
10.1007/bf00375376
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发表时间：
1985-01-01
期刊：
影响因子：
3.2
通讯作者：
CRESSWELL, P
中科院分区：
文献类型：
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作者：
SALTER, RD;HOWELL, DN;CRESSWELL, P
研究了人T淋巴母细胞样细胞系（LCL）和B淋巴母细胞样细胞系（LCL）杂交株中HLA I类和II类抗原表达的调控。T-LCL CEMR.3不表达HLA II类抗原。它表达少量总HLA I类抗原，不表达HLA- b抗原。B-LCL 721.174是一种因II类抗原表达缺失而免疫选择的辐射诱导变异。除了显示所有HLA-DR和DQ结构基因的缺失外，721.174不表达HLA-B抗原，与亲本细胞系相比，HLA-A抗原水平降低。721.174和CEMR.3的杂种表达由CEMR.3编码的II类抗原。721.174和CEMR.3编码的HLA I类抗原的表达也有所增加。具体地说，之前无法检测到的HLA-B5和HLA-Bw6抗原分别由721.174和CEMR.3编码。721.174编码的HLA-A2抗原表达增加。缺乏cemr .3衍生的6号染色体拷贝的杂交体的免疫选择变体失去了由721.174编码的HLA-B5抗原的表达，并且表达了HLA-A2的量减少。从这些数据推断，2个互补的反式作用因子介导HLA I类抗原在杂交株中的增强表达。其中一个因素是由位于6号染色体上的一个来自CEMR.3的基因提供的。第2个因子，由721.174引入，是先前被认为在CEMR.3与B-LCL杂交体中诱导CEMR.3编码的I类抗原表达的基因。
Regulation of HLA class I and class II antigen expression was studied in hybrids of human T and B lymphoblastoid cell lines (LCL). The T-LCL CEMR.3 expresses no HLA class II antigens. It expresses little total HLA class I antigen and no HLA-B antigens. The B-LCL 721.174 is a radiation-induced variant immunoselected for loss of class II antigen expression. In addition to showing a deletion of all HLA-DR and DQ structural genes, 721.174 expresses no HLA-B antigens and a decreased level of HLA-A antigen compared with the parental cell line. A hybrid of 721.174 and CEMR.3 expresses class II antigens encoded by CEMR.3. Increased expression of HLA class I antigens encoded by both 721.174 and CEMR.3 was also observed. Specifically, the previously undetectable HLA-B5 and HLA-Bw6 antigens encoded by 721.174 and CEMR.3, respectively, were present on the hybrid. INcreased expression of the HLA-A2 antigen encoded by 721.174 was also observed. An immunoselected variant of the hybrid lacking both CEMR.3-derived copies of chromosome 6 lost expression of the HLA-B5 antigen encoded by 721.174 and expressed a decreased amount of HLA-A2. From these data, it is inferred that 2 complementary trans-acting factors mediate enhanced expression of HLA class I antigens in the hybrid. One of these factors is provided by a gene located on chromosome 6 derived from CEMR.3. The 2nd factor, introduced by 721.174, is the gene previously postulated to induce expression of CEMR.3-encoded class I antigens in hybrids of CEMR.3 with B-LCL.