Analysis of competence in cultured sea urchin micromeres.
Analysis of competence in cultured sea urchin micromeres.
复制标题
养殖海胆微粒的能力分析。
DOI：
10.1016/0014-4827(92)90003-q
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发表时间：
1992
影响因子：
3.7
通讯作者：
Benson,S
中科院分区：
文献类型：
--
作者：
Page,L;Benson,S
海胆胚胎的微粒形成了初级间充质，即胚胎中的产精细胞。几乎没有什么是已知的性质和时间的胚胎线索，诱导或启动骨针形成的这些细胞。一个相关的问题是关于微粒细胞对线索做出反应的能力。为了检查在这个系统中的能力，我们已经暴露了培养的海胆micromeres的诱导培养基中含有马血清的不同时间段，并确定了一个时期，当micromeres有能力响应血清和形成骨针。受精后30至50小时的这一时间窗，对应于间充质细胞在体内聚集并开始形成合胞体的时间，在合胞体中将沉积骨针。50小时后感受态的丧失不是由于受损的细胞健康，因为此时的蛋白质合成与对照没有显著差异。同样地，在已经失去通过形成骨针对血清作出反应的能力的细胞中，仍然发生骨针基质mRNA（SM 50）和细胞表面糖蛋白（msp 130）的积累，这两个指标都是微球/间充质分化的指标。这些实验表明，这些细胞中感受态的获得和丧失是受调控的发育事件，并建立了一个体外系统，用于鉴定诱导信号识别和信号转导的分子基础。
Sea urchin embryo micromeres form the primary mesenchyme, the skeleton-producing cells of the embryo. Almost nothing is known about nature and timing of the embryonic cues which induce or initiate spicule formation by these cells. A related question concerns the competence of the micromeres to respond to the cues. To examine competence in this system we have exposed cultured sea urchin micromeres to an inducing medium containing horse serum for various periods of time and have identified a period when micromeres are competent to respond to serum and form spicules. This window, between 30 and 50 h after fertilization, corresponds to the time when mesenchyme cellsin vivoare aggregating and beginning to form the syncytium in which the spicule will be deposited. The loss of competence after 50 h is not due to impaired cell health since protein synthesis at this time is not significantly different from controls. Likewise the accumulation of a spicule matrix mRNA (SM 50) and a cell surface glycoprotein (msp 130), both indices of micromere/mesenchyme differentiation, still occurs in cells that have lost competence to respond to serum by forming spicules. These experiments demonstrate that the acquisition and loss of competence in these cells are regulated developmental events and establish anin vitrosystem for the identification of the molecular basis for inductive signal recognition and signal transduction.