Vitamin D--dependent calcium binding protein: immunocytochemical localization in chick kidney.
Vitamin D--dependent calcium binding protein: immunocytochemical localization in chick kidney.
复制标题
维生素 D 依赖性钙结合蛋白：鸡肾中的免疫细胞化学定位。
DOI：
10.1126/science.7025212
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Orci,L
中科院分区：
文献类型：
--
作者：
Roth,J;Thorens,B;Hunziker,W;Norman,AW;Orci,L
免疫细胞化学技术检测到鸡肾脏中维生素D依赖的钙结合蛋白仅定位于远曲小管、起始集合管和集合管的早期。这些肾小管段的插层细胞(富含线粒体)的钙结合蛋白为阴性。在亚细胞内，该蛋白存在于肾小管细胞的胞浆和胞核中。这些结果表明，维生素D依赖的钙结合蛋白在细胞内钙代谢中发挥作用，而不是直接参与鸟类肾脏膜介导的钙重吸收。
A vitamin D—dependent calcium binding protein in the chick kidney that was detected by immunocytochemical techniques was localized exclusively in the distal convoluted tubule, the initial collecting tubule, and the early part of the collecting tubule. The intercalated (mitochondria-rich) cells in these tubular segments were negative for the calcium binding protein. Subcellularly, the protein was found in the cytosol and the nucleus of the tubular cells. The results suggest a role for vitamin D—dependent calcium binding protein in intracellular calcium metabolism rather than a direct involvement in membrane-mediated calcium reabsorption in the avian kidney.