Antimicrobial susceptibility and molecular characterization of macrolide resistance of Mycoplasma bovis isolates from multiple provinces in China.
Antimicrobial susceptibility and molecular characterization of macrolide resistance of Mycoplasma bovis isolates from multiple provinces in China.
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DOI：
10.1292/jvms.15-0304
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Ma HX
中科院分区：
文献类型：
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作者：
Kong LC;Gao D;Jia BY;Wang Z;Gao YH;Pei ZH;Liu SM;Xin JQ;Ma HX
牛支原体通过动物运动在世界范围内广泛传播，已成为牛呼吸道疾病的重要病原体。但国内尚未研究抗菌药物对牛支原体的最低抑菌浓度。本研究的目的是确定中国呼吸道感染幼牛中分离出的牛支原体的流行率和抗生素耐药性。中国8个省肉牛养殖场32/45的牛呼吸道感染疫情中检出牛支原体。分离株对环丙沙星、恩诺沙星和多西环素敏感或具有中等敏感性，但经常对大环内酯类药物耐药（13/32，41%）。在对大环内酯类药物耐药的菌株中观察到位于 23S rRNA 结构域 V 的 rrnA 操纵子中的 A2058G（大肠杆菌编号）突变。 23S rRNA 结构域 V 中 rrnA 操纵子的这种单突变可能在牛支原体菌株对大环内酯类药物的耐药性中发挥重要作用。
Mycoplasma bovis has spread widely throughout the world via animal movement and has become an important pathogen of bovine respiratory disease. However, the minimum inhibitory concentrations of antimicrobials for Mycoplasma bovis have not been studied in China. The objective of this study was to determine the prevalence and antibiotic resistance of Mycoplasma bovis isolated from young cattle with respiratory infection in China. Mycoplasma bovis was detected in 32/45 bovine respiratory infection outbreaks at beef farms in 8 provinces in China. The isolates were susceptible or had medium sensitivity to ciprofloxacin, enrofloxacin and doxycycline, but were frequently resistant to macrolides (13/32, 41%). An A2058G (Escherichia coli Numbering) mutation located in the rrnA operon in domain V of 23S rRNA was observed in strains that were resistant to macrolides. This single mutations at the rrnA operon in domain V of 23S rRNA may play an important role in the resistance of Mycoplasma bovis strains to macrolides.