Separation of proteins and peptides by capillary electrochromatography in diol- and octadecyl-modified etched capillaries.
Separation of proteins and peptides by capillary electrochromatography in diol- and octadecyl-modified etched capillaries.
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DOI：
10.1016/s0021-9673(96)00864-3
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发表时间：
1997-02
期刊：
影响因子：
--
通讯作者：
J. Pesek;M. Matyska;L. Mauskar
中科院分区：
文献类型：
--
作者：
J. Pesek;M. Matyska;L. Mauskar
这项研究涉及一种毛细管电色谱方法的评估，该方法基于对熔融硅管内壁的蚀刻，随后通过硅烷化/硅氢化反应方案进行修饰。两种不同的有机部分，十八烷基和二醇，附着在蚀刻的毛细管壁上。这两个柱的性能与使用多肽(血管紧张素)和蛋白质样品的裸毛细管进行了比较。结果表明，对于十八烷基和二元醇修饰的毛细管，刻蚀过程充分增加了内壁的表面积，从而诱导了溶质键合相的相互作用。这两种修饰毛细管的分离能力不同，可能是由于两种配体的化学性质不同所致。与仅由于电泳迁移率效应而分离的裸毛细管相比，在相同的实验条件下，粘合蚀刻毛细管对相同溶质的分离因子也有显著差异。
This study involves the evaluation of a capillary electrochromatography method based on etching the inner walls of a fused-silica tube, which is subsequently modified by a silanization/hydrosilation reaction scheme. Two different organic moieties, octadecyl and diol, are attached to the etched capillary wall. The performance of these two columns is compared to a bare capillary using peptide (angiotensins) and protein samples. It is concluded that the etching process increases the surface area of the inner wall sufficiently to induce solute-bonded phase interactions for the capillaries modified with the octadecyl and diol moieties. The separation capabilities of the two modified capillaries are not the same, presumably due to differences in the chemical properties of the two ligands. When compared to a bare capillary where separation is due only to electrophoretic mobility effects, the bonded etched capillaries also exhibit significant differences in separation factors for the same solutes under identical experimental conditions.