Pioneer-like factor GAF cooperates with PBAP (SWI/SNF) and NURF (ISWI) to regulate transcription.
Pioneer-like factor GAF cooperates with PBAP (SWI/SNF) and NURF (ISWI) to regulate transcription.
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DOI：
10.1101/gad.341768.120
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发表时间：
2021-01-01
影响因子：
10.5
通讯作者：
Lis JT
中科院分区：
文献类型：
--
作者：
Judd J;Duarte FM;Lis JT
在这项研究中，Judd等人着手了解先驱因子如何将异染色质转化为活性染色质。使用RNAi和转录组学方法的组合，作者表明GAGA因子（GAF），一种果蝇先锋样因子，与SWI/SNF和ISWI家族染色质重塑因子一起发挥作用，以允许Pol II的募集并进入启动子近端暂停状态，并促进Pol II向生产性延伸的过渡。转录沉默基因必须在整个发育过程中被激活。这需要从启动子和增强子中去除核小体，以允许转录因子（TF）结合并募集辅激活因子和RNA聚合酶II（Pol II）。专门的先驱转录因子结合核小体包裹的DNA，通过尚不完全了解的机制执行染色质开放。在这里，我们表明，GAGA因子（GAF），果蝇先驱样因子，功能与SWI/SNF和ISWI家族染色质重塑，使招聘的Pol II和进入启动子近端暂停状态，并促进Pol II的过渡到生产性伸长。我们发现GAF与PBAP（SWI/SNF）相互作用以打开染色质并允许Pol II被募集。重要的是，该活性不依赖于先前提出的BNF;然而，GAF还与BNF在该过程的下游协同作用，以确保有效的Pol II暂停释放和过渡到生产性延伸，显然是通过其在精确定位+1核小体中的作用。这些结果证明了单个序列特异性先锋TF如何与重塑剂协同作用以激活基因组。此外，重塑的这种行为与酵母和小鼠中的发现一致，并且可能代表在整个真核生物中发现的一般保守机制。
In this study, Judd et al. set out to understand how pioneer factors transform heterochromatin to active chromatin. Using a combination of RNAi and transcriptomics methods, the authors show that GAGA factor (GAF), a Drosophila pioneer-like factor, functions with both SWI/SNF and ISWI family chromatin remodelers to allow recruitment of Pol II and entry to a promoter-proximal paused state, and also to promote Pol II's transition to productive elongation. Transcriptionally silent genes must be activated throughout development. This requires nucleosomes be removed from promoters and enhancers to allow transcription factor (TF) binding and recruitment of coactivators and RNA polymerase II (Pol II). Specialized pioneer TFs bind nucleosome-wrapped DNA to perform this chromatin opening by mechanisms that remain incompletely understood. Here, we show that GAGA factor (GAF), a Drosophila pioneer-like factor, functions with both SWI/SNF and ISWI family chromatin remodelers to allow recruitment of Pol II and entry to a promoter-proximal paused state, and also to promote Pol II's transition to productive elongation. We found that GAF interacts with PBAP (SWI/SNF) to open chromatin and allow Pol II to be recruited. Importantly, this activity is not dependent on NURF as previously proposed; however, GAF also synergizes with NURF downstream from this process to ensure efficient Pol II pause release and transition to productive elongation, apparently through its role in precisely positioning the +1 nucleosome. These results demonstrate how a single sequence-specific pioneer TF can synergize with remodelers to activate sets of genes. Furthermore, this behavior of remodelers is consistent with findings in yeast and mice, and likely represents general, conserved mechanisms found throughout eukarya.
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DOI：
10.1093/bioinformatics/btq033
发表时间：
2010-03-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Quinlan AR;Hall IM
通讯作者：
Hall IM
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
4.5
作者：
Fuda NJ;Guertin MJ;Sharma S;Danko CG;Martins AL;Siepel A;Lis JT
通讯作者：
Lis JT
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.9
作者：
Moshe, Arbel;Kaplan, Tommy
通讯作者：
Kaplan, Tommy