TRANSCRIPTIONAL REGULATOR OF OXIDATIVE STRESS-INDUCIBLE GENES - DIRECT ACTIVATION BY OXIDATION
TRANSCRIPTIONAL REGULATOR OF OXIDATIVE STRESS-INDUCIBLE GENES - DIRECT ACTIVATION BY OXIDATION
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DOI：
10.1126/science.2183352
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发表时间：
1990-04-13
期刊：
影响因子：
56.9
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
STORZ, G;TARTAGLIA, LA;AMES, BN
在鼠伤寒沙门氏菌和大肠杆菌中，oxyR基因正向调节氧化应激诱导的基因。OxyR蛋白的纯化表明，氧化而不是还原OxyR激活体外氧化应激诱导基因的转录。两种形式的OxyR之间的转换是快速和可逆的。氧化和还原形式的OxyR蛋白都能够结合到OxyR调节的启动子上游的三个不同序列，但这两种形式的OxyR与启动子区域的相互作用是不同的。结果表明，OxyR蛋白的直接氧化引起构象变化，OxyR通过该构象变化将氧化应激信号转导至RNA聚合酶。
The oxyR gene positively regulates gene induced by oxidative stress in Salmonella typhimurium and Escherichia coli. Purification of the OxyR protein showed that oxidized but not reduced OxyR activates transcription of oxidative stress-inducible genes in vitro. Conversion between the two forms of OxyR is rapid and reversible. Both the oxidized and the reduced forms of the OxyR protein are capable of binding to three diverse sequences upstream of OxyR-regulated promoters, but the interactions of the two forms of OxyR with the promoter regions are different. The results suggest that direct oxidation of the OxyR protein brings about a conformational change by which OxyR transduces an oxidative stress signal to RNA polymerase.