Core-shell silver nanoparticles for optical labeling of cells.
Core-shell silver nanoparticles for optical labeling of cells.
复制标题
用于细胞光学标记的核壳银纳米颗粒。
DOI：
10.1016/j.ab.2014.04.015
复制
发表时间：
2014
影响因子：
2.9
通讯作者：
Chumanov,George
中科院分区：
文献类型：
--
作者：
Dukes,KyleD;Christensen,KennethA;Chumanov,George
银纳米粒子已被修改与自组装单分子膜的羟基封端的长链硫醇和封装与二氧化硅壳。将所得核-壳纳米颗粒用作光学标记物，用于使用流式细胞术和显微镜进行细胞分析。纳米粒子中等离子体共振的激发导致可见光的强去偏振散射，从而允许在单个纳米粒子水平上进行检测。通过环氧化物-叠氮化物偶联化学用中性抗生物素蛋白修饰纳米颗粒，生物素化的靶向细胞表面受体的抗体结合到该纳米颗粒上。纳米颗粒标记物在具有高盐浓度的溶液中表现出长期稳定性，而没有聚集或银蚀刻。标记的细胞表现出两个数量级的散射强度增强与未标记的细胞相比。
Silver nanoparticles have been modified with self-assembled monolayers of hydroxyl-terminated long chain thiols and encapsulated with a silica shell. The resulting core–shell nanoparticles were used as optical labels for cell analysis using flow cytometry and microscopy. The excitation of plasmon resonances in nanoparticles results in strong depolarized scattering of visible light, permitting detection at the single nanoparticle level. The nanoparticles were modified with neutravidin via epoxide–azide coupling chemistry, to which biotinylated antibodies targeting cell surface receptors were bound. The nanoparticle labels exhibited long-term stability in solutions with high salt concentrations without aggregation or silver etching. Labeled cells exhibited two orders of magnitude enhancement of the scattering intensity compared with unlabeled cells.