MEGAKARYOBLASTIC DIFFERENTIATION OF PROERYTHROBLASTIC K562 CELL-LINE CELLS
MEGAKARYOBLASTIC DIFFERENTIATION OF PROERYTHROBLASTIC K562 CELL-LINE CELLS
复制标题
DOI：
10.1016/0145-2126(84)90143-7
复制
发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
BORNE, AEGKV
中科院分区：
文献类型：
--
作者：
TETTEROO, PAT;MASSARO, F;BORNE, AEGKV
用12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导人原红白血病细胞系K562向巨核细胞分化。当使用谱系特异性单克隆抗体监测TPA对K562细胞的作用时，检测到巨核细胞分化。针对GP [糖蛋白] IIIa上存在的表位的单克隆抗血小板抗体（C17）在诱导后似乎与K562细胞反应。这与红细胞特异性谱系抗原血型糖蛋白A的消失一起观察到。用血小板过氧化物酶（PPO）检测巨核细胞的超微结构。用C17抗体对细胞进行外标记和SDS[十二烷基硫酸钠]-聚丙烯酰胺凝胶电泳后，免疫沉淀证明TPA诱导的K562表达GP IIIa和GP IIb。针对血小板GP IIIa的另一个表位的单克隆抗体C15仅部分反应，或根本不反应，表明TPA诱导的K562上表达的GP IIIa在结构上不同于正常血小板上的GP IIIa。通过有限稀释和培养获得在所有细胞中表达血型糖蛋白A的K562克隆。当用TPA处理这些克隆时，再次获得巨核细胞。这些发现进行了讨论，在正常的巨核细胞生成。
The human proerythroblastic leukemia cell line K562 was induced to differentiate into megakaryocytic cells by 12-O-tetradecanoylphorbol-13-acetate (TPA). Megakaryocytic differentiation was detected when lineage-specific monoclonal antibodies were used to monitor the effect of TPA on K562 cells. A monoclonal anti-platelet antibody (C17) directed against an epitope present on GP [glycoprotein] IIIa appeared to react with K562 cells after induction. This was observed together with the disappearance of glycophorin A, the erythrocyte-specific lineage antigen. The induced megakaryocytic cells were also detected by ultrastructural platelet peroxidase (PPO). Immunoprecipitation, after ectolabeling of the cells with the C17 antibody and SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis, proved that TPA-induced K562 expressed both GP IIIa and GP IIb. The monoclonal antibody C15 directed against another epitope of platelet GP IIIa reacted only partially, or not at all, indicating that GP IIIa expressed on TPA-induced K562 differs structurally from that on normal platelets. K562 clones, expressing glycophorin A in all cells, were obtained by limiting dilution and culture. When these clones were treated with TPA, again megakaryocytic cells were obtained. These findings are discussed in relation to normal megakaryocytopoiesis.