Partial amino acid sequence of the heavy and light chains of botulinum neurotoxin type A.
Partial amino acid sequence of the heavy and light chains of botulinum neurotoxin type A.
复制标题
A型肉毒杆菌神经毒素重链和轻链的部分氨基酸序列。
DOI：
10.1016/0006-291x(84)90858-1
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
DasGupta,BR
中科院分区：
文献类型：
--
作者：
Schmidt,JJ;Sathyamoorthy,V;DasGupta,BR
双链（缺口）A型肉毒杆菌神经毒素是一种蛋白质（分子量为145,000），由一条重链和一条轻链（分子量分别为97,000和53,000）组成，它们通过二硫键连接在一起。我们在这里报告了轻链的前17个氨基酸残基和重链的前10个氨基酸残基的序列。用两种不同的方法从神经毒素中分离出重链，用唯一可行的方法分离出轻链。两种重链制剂的氨基酸序列相同。从两个单独制备的神经毒素批次中分离出的两个轻链样本也具有相同的序列。
The dichain (nicked) type A botulinum neurotoxin is a protein (mol. wt. 145,000) composed of a heavy and a light chain (mol. wt. 97,000 and 53,000, respectively) that are held together by disulfide bond(s). We report here the sequence of the first 17 amino acid residues of the light chain, and the first 10 residues of the heavy chain. The heavy chain was isolated from the neurotoxin by two different methods, while the light chain was isolated by the only available method. The identical amino acid sequence was found in both preparations of heavy chain. Two samples of the light chain isolated from two separately prepared batches of the neurotoxin also had identical sequences.