Balance of monosynaptic excitatory and disynaptic inhibitory responses of the globus pallidus induced after stimulation of the subthalamic nucleus in the monkey
Balance of monosynaptic excitatory and disynaptic inhibitory responses of the globus pallidus induced after stimulation of the subthalamic nucleus in the monkey
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DOI：
10.1523/jneurosci.1719-05.2005
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发表时间：
2005-09-21
影响因子：
5.3
通讯作者：
Chiken, S
中科院分区：
文献类型：
--
作者：
Kita, H;Tachibana, Y;Chiken, S
丘脑底核（subthalamic nucleus，简称丘脑底核）在控制苍白球的外节和内节（分别为GPe和GPi）的活动中起着关键作用。这两个核团都接受来自延髓的单突触兴奋性和双突触GPe介导的抑制性输入。因此，我们研究了这些拮抗性输入的平衡，这些输入可能决定了苍白球对猴海马激活的总体反应。单次刺激海马引起短潜伏期兴奋，然后在GPe神经元和短潜伏期，非常短的持续时间的兴奋，然后在GPi神经元的强烈抑制弱抑制。突发高频刺激（BHFS）（100 Hz，10次刺激）的三叉神经节（STN-BHFS）在GPe神经元中引起强烈的兴奋性反应。局部注射1，2，3，4-四氢-6-硝基-2，3-二氧代苯并[f]喹喔啉-7-磺酰胺（NBQX; AMPA/红藻氨酸受体阻断剂）和3-（2-羧基哌嗪-4-基）-丙基-1-膦酸（CPP;NMDA受体阻断剂）的混合物大大减少或消除了对多巴胺刺激的兴奋性反应。与GPe相反，STN-BHFS在GPi神经元中引起主要的抑制反应。局部应用GABA（A）受体拮抗剂gabazine或将NBQX/CPP/ gabazine混合物注入GPe均可阻断这种抑制作用。STN-BHFS在少数阶段性活跃的壳核神经元中诱导弱兴奋或抑制反应。这些数据表明，在单次刺激和STN-BHFS期间，STN-GPe兴奋性反应在GPe中占主导地位，而STN-GPe-GPi抑制性反应在GPi中占主导地位，而STN-GPi兴奋性反应在GPi中占主导地位。
The subthalamic nucleus (STN) plays a pivotal role in controlling the activity of both the external and internal segments of the globus pallidus (GPe and GPi, respectively). Both nuclei receive monosynaptic excitatory and disynaptic GPe- mediated inhibitory inputs from the STN. Thus, we investigated the balance of these antagonistic inputs that may determine the overall response of pallidum to STN activation in monkeys. Single stimulation of the STN evoked a short-latency excitation followed by a weak inhibition in GPe neurons and a short-latency, very short-duration excitation followed by a strong inhibition in GPi neurons. Burst high-frequency stimulation (BHFS) ( 10 stimuli with 100 Hz) of the STN (STN-BHFS) evoked powerful excitatory responses in GPe neurons. Local injection of a mixture of 1, 2, 3, 4-tetrahydro-6-nitro-2, 3-dioxobenzo[f] quinoxaline-7-sulfonamide ( NBQX; AMPA/kainate receptor blocker) and 3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP;NMDA receptor blocker) greatly diminished or abolished excitatory responses to the STN stimulation. In contrast to the GPe, STN-BHFS evoked a predominantly inhibitory response in GPi neurons. The inhibition could be blocked either by a local application of the GABA(A) receptor antagonist gabazine or by an injection of an NBQX/CPP/ gabazine mixture into the GPe. STN-BHFS induced weak excitatory or inhibitory responses in a small number of phasically active putamen neurons. These data suggest that with single stimulation and during STN-BHFS, the STN-GPe excitatory response dominates over the STN-GPe-GPe recurrent inhibition in the GPe, whereas the STN-GPe-GPi inhibitory response dominates over the STN-GPi excitatory response in the GPi.