Cell adhesion during bullet motion in capillaries
Cell adhesion during bullet motion in capillaries
复制标题
毛细血管中子弹运动期间的细胞粘附
DOI：
10.1152/ajpheart.00241.2016
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发表时间：
2016
影响因子：
4.8
通讯作者：
and Takuji Ishikawa
中科院分区：
文献类型：
--
作者：
Naoki Takeishi;Yohsuke Imai;Shunichi Ishida;Toshihiro Omori;Roger D. Kamm;and Takuji Ishikawa
数值分析的细胞粘附在毛细血管的直径是可比的或小于细胞。与之前大量关于白细胞和肿瘤细胞滚动的研究相反，关于毛细血管中细胞运动的研究仍有很多未知之处。细胞在流动中的固体和流体力学与配体-受体相互作用的滑移键模型相耦合。当毛细血管的尺寸减小时，细胞总是转变为“子弹状”运动，从而降低细胞的速度。得到了毛细管直径和受体密度的各种值的状态图。我们发现，即使配体-受体结合较弱，子弹运动也能使细胞牢固地粘附在毛细血管壁上。我们还量化了各种参数的影响，包括解离速率常数，弹簧常数，和细胞运动特性的反应性顺应性。我们的研究结果表明，即使在P-选择素糖蛋白配体-1（PSGL-1）和P-选择素（主要负责白细胞滚动）之间的相互作用下，细胞也能够在小毛细血管中显示出牢固的粘附。这些发现可能有助于理解白细胞堵塞和癌症转移等现象。
A numerical analysis is presented of cell adhesion in capillaries whose diameter is comparable to or smaller than that of the cell. In contrast to a large number of previous efforts on leukocyte and tumor cell rolling, much is still unknown about cell motion in capillaries. The solid and fluid mechanics of a cell in flow was coupled with a slip bond model of ligand-receptor interactions. When the size of a capillary was reduced, the cell always transitioned to “bullet-like” motion, with a consequent decrease in the velocity of the cell. A state diagram was obtained for various values of capillary diameter and receptor density. We found that bullet motion enables firm adhesion of a cell to the capillary wall even for a weak ligand-receptor binding. We also quantified effects of various parameters, including the dissociation rate constant, the spring constant, and the reactive compliance on the characteristics of cell motion. Our results suggest that even under the interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin, which is mainly responsible for leukocyte rolling, a cell is able to show firm adhesion in a small capillary. These findings may help in understanding such phenomena as leukocyte plugging and cancer metastasis.