Stereological and somatotopic analysis of the spinal microglial response to peripheral nerve injury
Stereological and somatotopic analysis of the spinal microglial response to peripheral nerve injury
复制标题
DOI：
10.1016/j.bbi.2006.10.017
复制
发表时间：
2007-07-01
影响因子：
15.1
通讯作者：
Salter, Michael W.
中科院分区：
文献类型：
--
作者：
Beggs, Simon;Salter, Michael W.
神经胶质和神经胶质-神经元信号在增强伤害性传递中的参与已经成为强烈的科学兴趣的领域。特别是，激活的小胶质细胞在周围神经损伤后神经性疼痛的发展和维持中的作用已经出现。在激活后，脊髓小胶质细胞增殖并释放许多能够调节脊髓内神经元兴奋性的物质。在这里，我们研究了脊髓小胶质细胞的反应，单侧备用神经损伤（SNI）的细胞数量的增加和空间分布的增加。设计为基础的体视学技术结合iba-1免疫组化，估计在幼稚和周围神经损伤的成年大鼠脊髓背角的小胶质细胞总数。此外，通过绘制后肢神经的中央末梢，绘制了小胶质细胞反应的躯体位置分布。SNI后，脊髓小胶质细胞数量显著增加：未处理大鼠坐骨神经背角区域中的小胶质细胞总数（平均值+/- SD）估计为28，591 +/- 2715。SNI后，小胶质细胞的数量为82，034 +/- 8828。虽然小胶质细胞激活的模式一般遵循躯体位置的边界，与大多数的小胶质细胞内的领土所占据的外周axotomised初级传入，一些蔓延被视为到区域所占据的完整的，“幸免”的腓肠神经的中央投影。这项研究提供了一个可重复的方法，测定脊髓小胶质细胞动力学周围神经损伤后，定量和空间。(c)2006年爱思唯尔公司All rights reserved.
The involvement of glia, and glia-neuronal signalling in enhancing nociceptive transmission has become an area of intense scientific interest. In particular, a role has emerged for activated microglia in the development and maintenance of neuropathic, pain following peripheral nerve injury. Following activation, spinal microglia proliferate and release many substances which are capable of modulating neuronal excitability within the spinal cord. Here, we the investigated the response of spinal microglia to a unilateral spared nerve injury (SNI) in terms of the quantitative increase in cell number and the spatial distribution of the increase. Design-based stereological techniques were combined with iba-1 immunohistochemistry to estimate the total number of microglia in the spinal dorsal horn in naive and peripheral nerve-injured adult rats. In addition, by mapping the central terminals of hindlimb nerves, the somatotopic distribution of the microglial response was mapped. Following SNI there was a marked increase in the number of spinal microglia: The total number of microglia (mean +/- SD) in the dorsal horn sciatic territory of the naive rat was estimated to be 28,591 +/- 2715. Following SNI the number of microglia was 82,034 +/- 8828. While the pattern of microglial activation generally followed somatotopic boundaries, with the majority of microglia within the territory occupied by peripherally axotomised primary afferents, some spread was seen into regions occupied by intact, 'spared' central projections of the sural nerve. This study provides a reproducible method of assaying spinal microglial dynamics following peripheral nerve injury both quantitatively and spatially. (c) 2006 Elsevier Inc. All rights reserved.