Cloning and Characterization of the Biosynthetic Gene Cluster for Tomaymycin, an SJG-136 Monomeric Analog
Cloning and Characterization of the Biosynthetic Gene Cluster for Tomaymycin, an SJG-136 Monomeric Analog
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DOI：
10.1128/aem.02325-08
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发表时间：
2009-05-01
影响因子：
4.4
通讯作者：
Gerratana, Barbara
中科院分区：
文献类型：
--
作者：
Li, Wei;Chou, ShenChieh;Gerratana, Barbara
由不产色链霉菌（Streptomyces achromogenes）产生的托马霉素是一种天然产生的吡咯并苯并二氮杂卓（PBD）。对该菌的生物合成基因簇进行了鉴定和测序。基因聚类分析揭示了一种新的生物合成途径的邻氨基苯甲酸部分的PBDs。基因置换和化学互补的研究被用来确认拟议的生物合成途径。
Tomaymycin produced by Streptomyces achromogenes is a naturally produced pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. The gene cluster analysis reveals a novel biosynthetic pathway for the anthranilate moiety of PBDs. Gene replacement and chemical complementation studies were used to confirm the proposed biosynthetic pathway.