Molecular beacons for isothermal fluorescence enhancement by the cleavage of RNase HII from Chlamydia pneumoniae
Molecular beacons for isothermal fluorescence enhancement by the cleavage of RNase HII from Chlamydia pneumoniae
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DOI：
10.1016/j.ab.2007.09.002
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发表时间：
2007-12-15
影响因子：
2.9
通讯作者：
Duan, Tao
中科院分区：
文献类型：
--
作者：
Hou, Jingli;Liu, Xipeng;Duan, Tao
本文介绍了一种荧光强度等温增强的新方法。该检测基于嵌合DNA- rn (1)-DNA分子信标（cMB）和靶DNA与肺炎衣原体RNase Fill （CpRNase HII）形成的双链物的切割。cMB的环序列是根据靶序列设计的，包含一个单一的核糖核苷酸。CpRNase HII切割与cMB （RHMB）结合，在相同数量的靶DNA存在下，荧光强度变化比杂交反应增加90倍。这些结果表明，在实时监测靶DNA时，RHMB法可以增强荧光信号。2007爱思唯尔公司版权所有。
This article describes a new assay for isothermal enhancement of fluorescence intensity. The assay is based on the cleavage of duplexes formed by the chimeric DNA-rN(1)-DNA molecular beacon (cMB) and target DNA with Chlamydia pneumoniae RNase Fill (CpRNase HII). The loop sequence of the cMB, which was designed according to the target sequence, contains a single ribonucleotide. The combination of CpRNase HII cleavage and cMB (RHMB) permitted a 90-fold increase in fluorescence intensity change compared with the hybridization reaction in the presence of the same amount of target DNA. These results indicate that the RHMB assay can enhance the fluorescence signal in real-time monitoring of the target DNA. 2007 Elsevier Inc. All rights reserved.