Oral immunization with influenza virus in biodegradable microspheres.
Oral immunization with influenza virus in biodegradable microspheres.
复制标题
使用可生物降解微球中的流感病毒进行口服免疫。
DOI：
10.1093/infdis/167.1.84
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
Mestecky,J
中科院分区：
文献类型：
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作者：
Moldoveanu,Z;Novak,M;Huang,WQ;Gilley,RM;Staas,JK;Schafer,D;Compans,RW;Mestecky,J
评价了聚合物微球作为流感病毒免疫的口服抗原递送系统。在口服或全身免疫BALB/c小鼠后，使用纯化的福尔马林灭活的A型流感病毒/H3 N2（包封在生物可降解和生物相容性微球中或游离在溶液中）比较获得的免疫应答。甲醛处理的流感疫苗在微囊化过程中保持了免疫原性，并且微囊化抗原在全身免疫后诱导的保护性免疫应答等于或高于常规疫苗诱导的保护性免疫应答。当经口给予致敏动物时，微囊化抗原诱导唾液中的抗流感抗体水平高于和血清中的抗流感抗体水平与通过全身免疫获得的抗流感抗体水平相当。此外，口服加强免疫对受活病毒攻击的动物提供了几乎完全的保护。
Polymeric microspheres were evaluated as an oral antigen delivery system for immunization with influenza virus. The immune responses obtained were compared after either oral or systemic immunization of BALB/c mice using purified, formalin-inactivated influenza virus type A/H3N2, either encapsulated in biodegradable and biocompatible microspheres or free in solution. The immunogenicity of formalin-treated influenza vaccine was preserved during the microencapsulation process, and the microencapsulated antigen induced protective immune responses after systemic immunization that were equal to or higher than those induced by conventional vaccine. When administered orally to primed animals, microencapsulated antigen induced levels of anti-influenza antibodies in saliva that were higher than and in serum that were comparable to those obtained by systemic immunization. Furthermore, oral booster immunization provided virtually complete protection of animals challenged with live virus.