Suppressive Potential of Paenibacillus Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato
Suppressive Potential of Paenibacillus Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato
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DOI：
10.1264/jsme2.me13172
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发表时间：
2014-07-19
影响因子：
2.2
通讯作者：
Tsushima, Seiya
中科院分区：
文献类型：
--
作者：
Sato, Ikuo;Yoshida, Shigenobu;Tsushima, Seiya
为筛选番茄根腐病生防候选菌株，研究了从番茄叶际分离的芽孢杆菌和类芽孢杆菌对番茄根腐病的抑制作用。利用番茄幼苗和盆栽番茄植株对20株细菌的抑菌活性进行了测定，筛选出2株类芽孢杆菌（Paenibacillus）菌株（12 HD 2和42 NP 7）作为生防候选菌株。在田间试验中，这两个菌株对病害具有抑制作用。扫描电子显微镜显示，经处理的细菌细胞定殖于根表面，并且当用菌株42 NP 7细胞处理幼苗的根时，细胞群在根上维持至少4周。虽然细菌菌株在体外对致病病原菌没有直接的抗真菌活性，但观察到用两种细菌菌株的细胞处理的番茄根的丙酮提取物的抗真菌活性增加。此外，RT-PCR分析证实，防御相关基因的表达诱导的根和叶片的细菌细胞处理的幼苗。因此，两种类芽孢杆菌属菌株的根定殖细胞被认为诱导番茄植物的抗性，这导致对疾病的抑制。
The suppressive potentials of Bacillus and Paenibacillus strains isolated from the tomato phyllosphere were investigated to obtain new biocontrol candidates against Fusarium crown and root rot of tomato. The suppressive activities of 20 bacterial strains belonging to these genera were examined using seedlings and potted tomato plants, and two Paenibacillus strains (12HD2 and 42NP7) were selected as biocontrol candidates against the disease. These two strains suppressed the disease in the field experiment. Scanning electron microscopy revealed that the treated bacterial cells colonized the root surface, and when the roots of the seedlings were treated with strain 42NP7 cells, the cell population was maintained on the roots for at least for 4 weeks. Although the bacterial strains had no direct antifungal activity against the causal pathogen in vitro, an increase was observed in the antifungal activities of acetone extracts from tomato roots treated with the cells of both bacterial strains. Furthermore, RT-PCR analysis verified that the expression of defense-related genes was induced in both the roots and leaves of seedlings treated with the bacterial cells. Thus, the root-colonized cells of the two Paenibacillus strains were considered to induce resistance in tomato plants, which resulted in the suppression of the disease.