Cloning and Characterization of Flagellin Genes and Identification of Flagellin Glycosylation from Thermophilic Bacillus Species
Cloning and Characterization of Flagellin Genes and Identification of Flagellin Glycosylation from Thermophilic Bacillus Species
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DOI：
10.1271/bbb.90092
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发表时间：
2009-06-01
影响因子：
1.6
通讯作者：
Ishizuka, Morio
中科院分区：
文献类型：
--
作者：
Hayakawa, Jumpei;Kondoh, Yoshihide;Ishizuka, Morio
在芽孢杆菌中鉴定出鞭毛蛋白的糖基化。PS3和嗜热脂肪芽孢杆菌。Fit活体互补实验表明，这些鞭毛蛋白基因不能恢复枯草芽孢杆菌鞭毛蛋白突变体的运动能力，而编码非糖基化鞭毛蛋白基因的枯草芽孢杆菌和芽孢杆菌。KPS3恢复运动。此外，在枯草芽孢杆菌中表达的四种鞭毛蛋白没有糖基化。我们推测，这些细菌的鞭毛细丝组装需要糖基化。
Flagellin glycosylation was identified in Bacillus sp. PS3 and Geobacillus stearothermophilus. fit vivo complementation showed that these flagellin genes did not restore the motility of a Bacillus subtilis flagellin mutant, whereas the genes encoding non-glycosylated flagellin from Geobacillus kaustophilus and Bacillus sp. Kps3 restored motility. Moreover, four types of flagellins expressed in B. subtilis were not glycosylated. We speculate that glycosylation is required for flagellar filament assembly of these bacilli.