Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells
Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells
复制标题
DOI：
10.3168/jds.2016-11729
复制
发表时间：
2017-03-01
影响因子：
3.5
通讯作者：
Bernabuccri, U.
中科院分区：
文献类型：
--
作者：
Basirico, L.;Morera, P.;Bernabuccri, U.
一些体外和体内研究已经证明了共轭亚油酸（CLA）异构体对氧化应激和脂质过氧化的保护作用。然而，只有少数相互矛盾的研究表明必需脂肪酸的抗氧化潜力。本研究的目的是通过体外研究比较CLA与其他必需脂肪酸对牛乳腺上皮细胞（BME-UV 1）巯基氧化还原状态的影响及其对乳腺氧化损伤的保护作用。用含有50 μ M顺式-9、反式-11 CLA、反式-10、顺式-12 CLA、α-t-亚麻酸、γ-亚麻酸和亚油酸的完全培养基处理BME-UV 1细胞。为了评估细胞的抗氧化反应，谷胱甘肽，NADPH，和γ-谷氨酰-半胱氨酸连接酶的活性测定后48小时的脂肪酸（FA）。细胞内活性氧和丙二醛的产生也进行了评估补充FA的细胞。评估暴露于H2O（2）3小时后活性氧的产生，以评估和比较不同FA对H2O（2）诱导的氧化应激的潜在保护作用。所有FA处理诱导细胞内GSH增加，与高浓度的NADPH和γ-谷氨酰-半胱氨酸连接酶活性增加相匹配。补充FA的细胞显示细胞内丙二醛水平降低。特别是，CLA异构体和亚油酸补充剂显示出更好的抗氧化细胞反应对氧化损伤诱导的11202相比，其他FA。
Some in vitro and in vivo studies have demonstrated protective effects of conjugated linoleic acid (CLA) isomers against oxidative stress and lipid peroxidation. However, only a few and conflicting studies have been conducted showing the antioxidant potential of essential fatty acids. The objectives of the study were to compare the effects of CLA to other essential fatty acids on the thiol redox status of bovine mammary epithelia cells (BME-UV1) and their protective role against oxidative damage on the mammary gland by an in vitro study. The BME-UV1 cells were treated with complete medium containing 50 mu M of cis-9,trans-11 CLA, trans-10, cis-12 CLA, alpha t-linolenic acid, gamma-linolenic acid, and linoleic acid. To assess the cellular antioxidant response, glutathione, NADPH, and gamma-glutamyl-cysteine ligase activity were measured 48 h after addition of fatty acids (FA). Intracellular reactive oxygen species and malondialdehyde production were also assessed in cells supplemented with FA. Reactive oxygen species production after 3 h of H2O (2)exposure was assessed to evaluate and to compare the potential protection of different FA against H2O (2)-induced oxidative stress. All FA treatments induced an intracellular GSH increase, matched by high concentrations of NADPH and an increase of gamma-glutamyl-cysteine ligase activity. Cells supplemented with FA showed a reduction in intracellular malondialdehyde levels. In particular, CLA isomers and linoleic acid supplementation showed a better antioxidant cellular response against oxidative damage induced by 11202 compared with other FA.