Mechanical characterization of an in-body tissue-engineered autologous collagenous sheet for application as an aortic valve reconstruction material.
Mechanical characterization of an in-body tissue-engineered autologous collagenous sheet for application as an aortic valve reconstruction material.
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作为主动脉瓣重建材料应用的体内组织工程自体胶原片的机械特性。
DOI：
10.1016/j.jbiomech.2019.109528
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Nakayama Y.
中科院分区：
文献类型：
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作者：
Terazawa T;Takayuki Kawashima T;Tadashi Umeno T; Wada T;Ozaki S;Miyamoto S;Nakayama Y.
使用戊二醛处理的自体心包重建主动脉瓣被称为“主动脉瓣新尖化”（AVNeo）。体内组织构建（In-body tissue architecture，iBTA）是一种无细胞的体内组织工程技术，其可以通过皮下包埋特别设计的模具形成所需形状的自体可植入组织，用于制备称为“生物片”的片状胶原组织。将具有以交替（n = 30）或平行（n = 36）模式排列的几个线狭缝的圆柱形模具皮下包埋在山羊（n = 12）中2或3个月。干燥在模具中形成的管状组织，然后沿纵向切割，从而获得Biosheets（5 × 7 cm）。使用交替模式模具的成功率为97.6%，平行模具的成功率为97.2%。Biosheets的厚度绘图显示，除线投影部分外，其整个表面光滑，无任何缺陷。平均壁厚可控制在约100 μ m的范围内。通过改变模具中的差距的大小（0.75-1.5 mm），交替狭缝图案的生物片被认为是几乎各向同性的机械性能（极限拉伸强度，断裂应变，和杨氏模量）。尽管生物片壁的组成在其密度方面是不均匀的（其随厚度而变化），但在临床上使用的戊二醛处理的心包作为对照的厚度范围内，所有交替图案化生物片的断裂强度几乎随厚度线性增加，并且大于人主动脉瓣小叶的断裂强度。因此，交替图案生物片有可能用于AVNeo的替代主动脉瓣叶材料。
The reconstruction of the aortic valve using glutaraldehyde-treated autologous pericardium is known as “aortic valve neo-cuspidization” (AVNeo). In-body tissue architecture (iBTA), a cell-free, in vivo tissue-engineering technology that can form autologous implantable tissues of the desired shape by subcutaneous embedding specially designed molds, was used to prepare sheet-like collagenous tissues called “Biosheets”. Cylindrical molds with several line slits arranged in an alternating (n = 30) or parallel (n = 36) pattern were subcutaneously embedded in goats (n = 12) for 2 or 3 months. The tubular tissues formed in the molds were dried and then cut in the longitudinal direction, thus obtaining Biosheets (5 × 7 cm). The success rate was 97.6% when using the alternating-pattern molds and 97.2% for the parallel molds. Thickness mapping of the Biosheets showed that their entire surface, except for the line-projection portions, was smooth without any defects. The average wall thickness could be controlled over a range of ca. 0.2–0.5 mm by changing the size of the gap (0.75–1.5 mm) in the molds. The alternating slit-patterned Biosheets were found to be almost isotropic in their mechanical properties (ultimate tensile strength, fracture strain, and Young’s modulus). Although the composition of the Biosheet wall was heterogeneous in terms of its density (which varied with the thickness), the breaking strength of all the alternating-patterned Biosheets increased almost linearly with the thickness within the range of the thickness of clinically used glutaraldehyde-treated pericardium as a control, and was larger than that of human aortic valve leaflets. Therefore, the alternating-patterned Biosheets have potential for use in an alternative aortic leaflet material in AVNeo.