Inhibition of motor axon growth by T-cadherin substrata.
Inhibition of motor axon growth by T-cadherin substrata.
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DOI：
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发表时间：
1996-10
期刊：
影响因子：
4.6
通讯作者：
B. Fredette;J. Miller;B. Ranscht
中科院分区：
文献类型：
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作者：
B. Fredette;J. Miller;B. Ranscht
当脊髓运动神经元投射到它们的后肢目标时，它们的生长锥会避开沿着的特定区域。T-钙粘蛋白离散地分布在避开的尾侧巩膜和突触外肌肉表面（B. J. Fredette和B。Ranscht（1994）J. Neurosci. 14，7331-7346），因此，在体外测试了T-钙粘蛋白抑制神经突生长的能力。T-钙粘蛋白抑制神经突起的延伸从选择神经元群体作为一个基质，并作为一种可溶性重组蛋白。抗T-钙粘蛋白抗体中和抑制。脊髓运动神经元仅在轴突生长穿过硬节和肌肉神经支配的阶段受到抑制。抑制反应对应于神经元T-钙粘蛋白的表达，这表明了一种嗜同性结合机制。这些结果表明，T-cadherin是一个负的运动轴突投射的指导线索。
As spinal motor neurons project to their hindlimb targets, their growth cones avoid particular regions along their pathway. T-cadherin is discretely distributed in the avoided caudal sclerotome and on extrasynaptic muscle surfaces (B. J. Fredette and B. Ranscht (1994) J. Neurosci. 14, 7331-7346), and therefore, the ability of T-cadherin to inhibit neurite growth was tested in vitro. T-cadherin inhibited neurite extension from select neuron populations both as a substratum, and as a soluble recombinant protein. Anti-T-cadherin antibodies neutralized the inhibition. Spinal motor neurons were inhibited only during the stages of axon growth across the sclerotome and muscle innervation. Inhibitory responses corresponded to neuronal T-cadherin expression, suggesting a homophilic binding mechanism. These results suggest that T-cadherin is a negative guidance cue for motor axon projections.