OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand
OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand
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DOI：
10.1002/eji.1830260805
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发表时间：
1996-08-01
影响因子：
5.4
通讯作者：
Barclay, AN
中科院分区：
文献类型：
--
作者：
AlShamkhani, A;Birkeland, ML;Barclay, AN
OX40是肿瘤坏死因子（TNF）受体/神经生长因子（NGF）受体超家族的一员，首次被MRC OX40单克隆抗体（mAb）鉴定为激活大鼠CD4（+）细胞的标记物。最近发现了OX40的配体（称为OX40配体或OX40L），其序列与TNF相似。小鼠ox40l -免疫球蛋白融合蛋白（OX40L-Ig）结合活化小鼠CD4（+）和CD8（+）细胞（Baum， P. R .等，EMBO J. 1994）。[13:39 . 92]提示OX40可能在小鼠和大鼠T细胞上有不同的表达模式。然而，这并不能排除CD8（+）细胞上存在另一种与OX40L结合的受体。我们比较了MRC OX40 mAb与OX40L-Ig与活化的大鼠淋巴结细胞的结合，发现两者识别相同的蛋白，即OX40， OX40在CD4（+）和CD4(+) CD8 α(+)细胞上表达，而在CD4(-) CD8（+）细胞上不表达。我们利用重组小鼠OX40蛋白构建了一种新的单抗（MRC OX86），并通过双色流式细胞术证实小鼠OX40在CD4和CD8单阳性细胞上表达。此外，与MRC OX40单抗不同，新的MRC OX86单抗不阻断OX40L的结合。我们得出结论，OX40在活化的小鼠和大鼠T细胞上差异表达，并且是OX40L的唯一受体。
OX40, a member of the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF) receptor superfamily was first identified as a marker of activated rat CD4(+) cells with the MRC OX40 monoclonal antibody (mAb). A ligand for OX40 (called OX40 ligand or OX40L) has recently been identified and has sequence similarity to TNF. Mouse OX40L-immunoglobulin fusion protein (OX40L-Ig) binds to activated mouse CD4(+) and CD8(+) cells (Baum, P. R, et al., EMBO J. 1994. 13: 3992) suggesting that OX40 could have a differential pattern of expression on mouse and rat T cells. This, however, did not rule out the presence of an alternative receptor on CD8(+) cells that also binds the OX40L. We have compared the binding of the MRC OX40 mAb with that of OX40L-Ig to activated rat lymph node cells and show that both recognize the same protein, namely OX40 which is expressed on CD4(+) and CD4(+) CD8 alpha(+) cells, but not on CD4(-) CD8(+) cells. We have raised a new mAb (MRC OX86) using recombinant mouse OX40 protein and show by two-color flow cytometry that mouse OX40 is expressed on CD4 and CD8 single-positive cells. In addition, the new MRC OX86 mAb, unlike the MRC OX40 mAb, did not block binding of the OX40L. We conclude that OX40 is differentially expressed on activated mouse and rat T cells and is the sole receptor for the OX40L.