Multiplexed detection of nucleic acids using 19F NMR chemical shift changes based on DNA photo-cross-linking of 3-vinylcarbazole derivatives
Multiplexed detection of nucleic acids using 19F NMR chemical shift changes based on DNA photo-cross-linking of 3-vinylcarbazole derivatives
复制标题
基于 3-乙烯基咔唑衍生物 DNA 光交联，使用 19F NMR 化学位移变化多重检测核酸
DOI：
10.1039/c7ob03008h
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发表时间：
2018
影响因子：
3.2
通讯作者：
Fujimoto Kenzo
中科院分区：
文献类型：
--
作者：
Nakamura Shigetaka;Takashima Yasuharu;Fujimoto Kenzo
核酸的检测方法是分析生物系统和诊断疾病的有用工具。我们证明了通过超快DNA光交联和3-乙烯基咔唑衍生物取代的影响，基于大的化学位移检测任何核酸的可行性。这些化学位移使得能够使用杂交链反应对任何链进行序列特异性检测。
The detection methodology for nucleic acids is a useful tool for the analysis of biological systems and diagnosis of diseases. We demonstrated the feasibility of the detection of any nucleic acids based on large chemical shifts via ultrafast DNA photo-cross-linking and the effects of substitution by 3-vinylcarbazole derivatives. These chemical shifts enable the sequence-specific detection of any strand using hybridization chain reaction.