Protective T-cell-based immunity induced in neonatal mice by a single replicative cycle of herpes simplex virus
Protective T-cell-based immunity induced in neonatal mice by a single replicative cycle of herpes simplex virus
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DOI：
10.1128/jvi.75.1.83-89.2001
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发表时间：
2001-01-01
影响因子：
5.4
通讯作者：
Suter, M
中科院分区：
文献类型：
--
作者：
Franchini, M;Abril, C;Suter, M
新生儿对感染非常敏感，因为他们的免疫系统尚未完全发育，对抗原暴露的反应优先，无反应性。紫外线灭活的单纯疱疹病毒1型（HSV-1）代表了这样的抗原，不会诱导新生儿的免疫应答。相比之下，保护性T细胞在新生小鼠中通过在出生后24小时内给予一次DISC HSV-1的单个复制周期引发。在野生型或干扰素缺陷型小鼠中诱导的HSV-1致敏的CD 4（+）或CD 8（+）T细胞中的每一种都赋予暴露于致死病毒攻击的幼稚动物抗性。以高达DISC HSV-1的10（4）倍的可变剂量注射灭活HSV-1，在新生儿中诱导任何可检测的免疫应答方面无效。因此，HSV-1复制一次的能力，而不是病毒颗粒本身的数量，在新生小鼠中诱导保护性T细胞相关免疫中起决定性作用。
Newborns are very susceptible to infections because their immune systems are not fully developed and react to antigen exposure preferentially with unresponsiveness. UV-inactivated herpes simplex virus type 1 (HSV-1) represents such an antigen and does not induce an immune response in neonates. In contrast, protective T cells were primed in newborn mice by a single replicative cycle of DISC HSV-1 given once within 24 h of birth. Each of the HSV-l-primed CD4(+) or CD8(+) T cells induced in wild-type or interferon-deficient mice conferred resistance to naive animals exposed to a lethal virus challenge. Inactivated HSV-1, injected at variable doses up to 10(4) times that of DISC HSV-1, was ineffective in inducing any detectable immune responses in neonates. Thus, the capacity of HSV-1 to replicate once, but not the number of virus particles per se, was decisive in inducing protective T-cell-associated immunity in newborn mice.