New graft inserter for descemet stripping automated endothelial keratoplasty.
New graft inserter for descemet stripping automated endothelial keratoplasty.
复制标题
用于后弹力层剥离自动内皮角膜移植术的新型移植插入器。
DOI：
10.1097/ico.0000000000000073
复制
发表时间：
2014
期刊：
影响因子：
2.8
通讯作者：
Amano S
中科院分区：
文献类型：
--
作者：
Toyama T;Honda N;Usui T;Amano S
目的：介绍一种单手即可使用的新型植片植入器，用于剥离式全自动角膜内皮移植，并用该植片在体外兔模型上检测植片植入后内皮细胞的丢失情况。方法：用飞秒激光制备兔角膜移植片(∼150μm)。移植物被插入人工前房，通过拉出技术，使用Busin滑动或推出技术，使用我们的新的插入器。用台盼蓝和茜素红S染色评价ECL。结果：对照组ECL面积为18.7±1.79%，Busin滑动组为25.4±2.32%，新插入组为24.7±1.56%。Busin Glide组和新插入物组内皮细胞的平均面积百分比均显著高于对照组(P=0.001)。结论：在Descemet剥离自动内皮角膜移植术中，我们的新型插入器可能为供体组织的植入提供了一种有利的选择。
Purpose:The aim of this study was to introduce a newly developed graft inserter for Descemet stripping automated endothelial keratoplasty, which can be readily used with 1 hand, and to examine endothelial cell loss (ECL) after graft insertion using this new inserter in an ex vivo rabbit model.Methods:Rabbit corneal grafts (∼ 150 μm) were prepared by using a femtosecond laser. The grafts were inserted into an artificial anterior chamber by the pull-through technique using a Busin glide or the push-out technique using our new inserter. ECL was evaluated by means of trypan blue and alizarin red S staining. Grafts that were not injected served as a control group.Results:The mean percentages of ECL area in the control, Busin glide, and new inserter groups were 18.7±1.79%, 25.4±2.32%, and 24.7±1.56%, respectively. The mean percentages of the ECL area in both the Busin glide group and new inserter groups were significantly greater than that in the control group (P= 0.001). There was no statistically significant difference between the Busin glide group and the new inserter group.Conclusions:Our new inserter may provide a favorable option for donor tissue insertion in Descemet stripping automated endothelial keratoplasty procedures.