Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue
Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue
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DOI：
10.1038/s41598-020-69368-2
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发表时间：
2020-07-27
影响因子：
4.6
通讯作者：
Nakano, Kazuhiko
中科院分区：
文献类型：
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作者：
Nomura, Ryota;Kadota, Tamami;Nakano, Kazuhiko
据推测，幽门螺杆菌在儿童时期通过口腔感染胃组织，但口腔中幽门螺杆菌感染的危险因素尚不清楚。在这项研究中，我们分析了变形链球菌（一种主要的致龋菌）对幽门螺杆菌在口腔和胃组织定植的影响。断奶期的大鼠在口腔中感染变形链球菌，然后喂食诱发龋齿的饮食以促进变形链球菌定植。感染变形链球菌1个月后，大鼠口腔内感染幽门螺杆菌；然后在幽门螺杆菌感染后1个月对大鼠实施安乐死。在同时感染变形链球菌和幽门螺杆菌的大鼠的口腔中检测到幽门螺杆菌，但在单独感染幽门螺杆菌的大鼠中未检测到幽门螺杆菌。此外，在同时感染变形链球菌和幽门螺杆菌的大鼠中观察到幽门螺杆菌在胃组织中的定植和典型的胃肠道损伤。当幽门螺杆菌与变形链球菌形成的体外生物膜共培养时，大量幽门螺杆菌侵入变形链球菌形成的生物膜。我们的结果表明变形链球菌参与幽门螺杆菌感染的建立。
Helicobacter pylori is presumed to infect gastric tissue via the oral cavity in childhood, whereas risk factors for H. pylori infection in the oral cavity are unknown. In this study, we analysed the effects of Streptococcus mutans, a major cariogenic bacterial species, on H. pylori colonisation in the oral cavity, as well as gastric tissue. Rats in the weaning period were infected with S. mutans in the oral cavity, then fed a caries-inducing diet to facilitate S. mutans colonisation. One month after S. mutans infection, rats were infected with H. pylori in the oral cavity; rats were then euthanised at 1 month after H. pylori infection. H. pylori was detected in the oral cavities of rats infected with both S. mutans and H. pylori, but not in rats infected with H. pylori alone. In addition, H. pylori colonisation in the gastric tissue and typical gastrointestinal damage were observed in rats infected with both S. mutans and H. pylori. When H. pylori was co-cultured with in vitro biofilm formed by S. mutans, a large number of H. pylori bacteria invaded the biofilm formed by S. mutans. Our results suggest that S. mutans is involved in the establishment of H. pylori infection.