ASSESSMENT OF BRAIN-TUMOR CELL MOTILITY IN-VIVO AND IN-VITRO
ASSESSMENT OF BRAIN-TUMOR CELL MOTILITY IN-VIVO AND IN-VITRO
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DOI：
10.3171/jns.1995.82.4.0615
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发表时间：
1995-04-01
影响因子：
4.1
通讯作者：
SILBERGELD, DL
中科院分区：
文献类型：
--
作者：
CHICOINE, MR;SILBERGELD, DL
脑肿瘤远离原发肿瘤的扩散有助于疾病进展，在某些情况下，甚至主导疾病进展。采用三种方法对脑肿瘤细胞在体内和体外的运动特性进行了表征：1)植入大鼠大脑皮层2周后，单个带有荧光标记的C6细胞已迁移到距离肿瘤体16 mm以上的脑部位；2)延时视频显微镜观察脑肿瘤细胞的运动能力为12.5 μ m/hr。在恶性程度较高的细胞中，褶皱前缘和假足形成最为精细；3)设计了一种体外实验，定量评价从高细胞密度区域到低细胞密度区域的运动性。将人脑肿瘤细胞置于培养皿中央，洗涤后再冷冻，在培养皿中心建立一个2厘米的圆形细胞区。运动是通过每天在距离中心区域周长的预定距离上计数细胞来确定的。24小时发现细胞距周长1厘米，4天发现细胞距周长3厘米。血药浓度升高，运动能力增强；然而，纤维连接蛋白和羟基脲在G(0)期阻滞细胞都不会改变其运动性。细胞松弛素B的加入阻止了细胞骨架的组装，从而阻止了细胞的运动。活力随着恶性肿瘤的增加而增加。细胞亚群是通过克隆扩增的细胞迁移最快的盘子外围。虽然与原始细胞系相比，形态上难以区分，但这些亚群表现出显著增加的运动性。
Brain tumor dispersal far from bulk tumor contributes to and, in some instances, dominates disease progression. Three methods were used to characterize brain tumor cell motility in vivo and in vitro: 1) 2 weeks after implantation in rat cerebral cortex, single C6 cells labeled with a fluorescent tag had migrated to brain sites greater than 16 mm distant from bulk tumor; 2) time-lapse videomicroscopy of human brain tumor cells revealed motility of 12.5 mu m/hr. Ruffling leading edges and pseudopod formation were most elaborate in more malignant cells; 3) an in vitro assay was devised to quantitatively evaluate motility from a region of high cell density to one of lower cell density.Human brain tumor cells were plated in the center of a petri dish, washed, and refed, establishing a 2-cm circular zone of cells in the dish center. Motility was determined by counting cells daily at predetermined distances from the central zone perimeter. Cells were found 1 cm from the perimeter by 24 hours and 3 cm from the perimeter by 4 days. Increasing serum concentration increased motility; however, neither fibronectin nor arrest of cells in the G(0) phase by hydroxyurea altered motility. The addition of cytochalasin B to block cytoskeletal assembly prevented cell motility. Motility increased with increased malignancy. Subpopulations of cells were created by clonal amplification of cells that had migrated most rapidly to the dish periphery. Although morphologically indistinguishable when compared to the original cell line from which they were derived, these subpopulations demonstrated significantly increased motility.