Molecular cloning and characterization of first organic matrix protein from sclerites of red coral, Corallium rubrum.
Molecular cloning and characterization of first organic matrix protein from sclerites of red coral, Corallium rubrum.
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DOI：
10.1074/jbc.m112.352005
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发表时间：
2012-06-01
期刊：
影响因子：
--
通讯作者：
Tambutté S
中科院分区：
文献类型：
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作者：
Debreuil J;Tambutté E;Zoccola D;Deleury E;Guigonis JM;Samson M;Allemand D;Tambutté S
背景：Alcyonarian 物种的骨片是由捕获在矿物无机部分内的有机基质分子形成的生物矿物。结果：巩膜素是一种小的碱性有机基质蛋白，由成硬化细胞合成并掺入巩膜中。结论：巩膜素是第一个完全表征并定位于红色 C. rubrum 巩膜中的有机基质蛋白。意义：巩膜蛋白提供了有关红色 C. rubrum 生物矿化途径的信息。我们在这里首次报道了从红珊瑚红珊瑚骨片的有机基质（OM）中分离和表征蛋白质。这种名为 scleritin 的蛋白质是从 OM 的 EDTA 可溶部分中提取的主要蛋白质之一。通过将来自 de novo 质谱和 Edman 降解的氨基酸序列与 C. rubrum 的表达序列标签库数据集进行比较，获得了整个开放阅读框 (ORF)。巩膜素是一种分泌型碱性磷酸化蛋白，具有 135 个氨基酸的短氨基酸序列和 20 个氨基酸的信号肽。从针对巩膜素肽序列产生的特异性抗体，我们获得了成硬化细胞的免疫标记和巩膜的 OM，这提供了有关红色 C. rubrum 中生物矿化途径的信息。
Background: Sclerites of alcyonarian species are biominerals formed of organic matrix molecules trapped inside a mineral inorganic fraction. Results: Scleritin is a small basic organic matrix protein, synthetized by scleroblasts and incorporated into sclerites. Conclusion: Scleritin is the first organic matrix protein fully characterized and localized in the sclerites of C. rubrum. Significance: Scleritin provides information on the biomineralization pathway in C. rubrum. We report here for the first time the isolation and characterization of a protein from the organic matrix (OM) of the sclerites of the alcyonarian, Corallium rubrum. This protein named scleritin is one of the predominant proteins extracted from the EDTA-soluble fraction of the OM. The entire open reading frame (ORF) was obtained by comparing amino acid sequences from de novo mass spectrometry and Edman degradation with an expressed sequence tag library dataset of C. rubrum. Scleritin is a secreted basic phosphorylated protein which exhibits a short amino acid sequence of 135 amino acids and a signal peptide of 20 amino acids. From specific antibodies raised against peptide sequences of scleritin, we obtained immunolabeling of scleroblasts and OM of the sclerites which provides information on the biomineralization pathway in C. rubrum.