Virulence and draft genome sequence overview of multiple strains of the swine pathogen Haemophilus parasuis.
Virulence and draft genome sequence overview of multiple strains of the swine pathogen Haemophilus parasuis.
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DOI：
10.1371/journal.pone.0103787
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Phillips GJ
中科院分区：
文献类型：
--
作者：
Brockmeier SL;Register KB;Kuehn JS;Nicholson TL;Loving CL;Bayles DO;Shore SM;Phillips GJ
副猪嗜血杆菌是引起猪格莱瑟病的病原，其特征是全身感染，导致多发性浆膜炎、脑膜炎和关节炎。这种动物疾病的调查是复杂的巨大差异，疾病的严重程度所造成的H。副猪嗜血杆菌菌株，范围从致命的全身性疾病亚临床携带。为了确定基因型的差异，可以解释毒力表型，我们建立了毒力，并进行全基因组序列分析，11 H。副猪型菌株通过评价剖腹产、初乳剥夺（CDCD）猪鼻内攻毒后的发病率和死亡率来评估毒力。来自菌株长崎的基因组DNA（血清型5），12939（血清型1），SW 140（血清型2），29755（血清型5），MN-H（血清型13），84-15995（血清型15），SW 114使用H465（血清型3）、H465（血清型11）、D 74（血清型9）和174（血清型7）来产生用于基因组测序和从头组装的Illumina配对末端文库。H.副猪嗜血杆菌长崎、12939、SH 0165（血清型5）、SW 140、29755和MN-H菌株表现出高水平的毒力。尽管这些组之间的疾病表达存在微小差异，但用这些菌株攻毒的所有猪在攻毒后1-7天内均出现与Glässer病一致的临床体征。H.副猪嗜血杆菌菌株84-15995和SW 114是中等毒性的，因为用每种菌株感染的猪中约有一半发生了格拉瑟病。H.副猪链球菌菌株H465、D 74和174在⑶ CD猪模型中是最低毒力或无毒力的。菌株之间的比较基因组分析确定了编码区的几个值得注意的差异。这些编码区包括预测的外膜、代谢和菌毛蛋白或粘附素相关基因，其中一些可能导致攻毒后观察到的毒力和全身性疾病差异。这些数据将有助于鉴别H.副猪嗜血杆菌毒力因子和疫苗靶点。
Haemophilus parasuis is the cause of Glässer's disease in swine, which is characterized by systemic infection resulting in polyserositis, meningitis, and arthritis. Investigation of this animal disease is complicated by the enormous differences in the severity of disease caused by H. parasuis strains, ranging from lethal systemic disease to subclinical carriage. To identify differences in genotype that could account for virulence phenotypes, we established the virulence of, and performed whole genome sequence analysis on, 11 H. parasuis strains. Virulence was assessed by evaluating morbidity and mortality following intranasal challenge of Caesarean-derived, colostrum-deprived (CDCD) pigs. Genomic DNA from strains Nagasaki (serotype 5), 12939 (serotype 1), SW140 (serotype 2), 29755 (serotype 5), MN-H (serotype 13), 84-15995 (serotype 15), SW114 (serotype 3), H465 (serotype 11), D74 (serotype 9), and 174 (serotype 7) was used to generate Illumina paired-end libraries for genomic sequencing and de novo assembly. H. parasuis strains Nagasaki, 12939, SH0165 (serotype 5), SW140, 29755, and MN-H exhibited a high level of virulence. Despite minor differences in expression of disease among these groups, all pigs challenged with these strains developed clinical signs consistent with Glässer's disease between 1–7 days post-challenge. H. parasuis strains 84-15995 and SW114 were moderately virulent, in that approximately half of the pigs infected with each developed Glässer's disease. H. parasuis strains H465, D74, and 174 were minimally virulent or avirulent in the CDCD pig model. Comparative genomic analysis among strains identified several noteworthy differences in coding regions. These coding regions include predicted outer membrane, metabolism, and pilin or adhesin related genes, some of which likely contributed to the differences in virulence and systemic disease observed following challenge. These data will be useful for identifying H. parasuis virulence factors and vaccine targets.