FURTHER-STUDIES ON QUANTIFYING PHOTOSYSTEM-II INVIVO BY FLASH-INDUCED OXYGEN YIELD FROM LEAF-DISKS
FURTHER-STUDIES ON QUANTIFYING PHOTOSYSTEM-II INVIVO BY FLASH-INDUCED OXYGEN YIELD FROM LEAF-DISKS
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DOI：
10.1071/pp9910397
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发表时间：
1991-01-01
期刊：
影响因子：
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通讯作者：
ANDERSON, JM
中科院分区：
文献类型：
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作者：
CHOW, WS;HOPE, AB;ANDERSON, JM
它被简单地显示[W。S. Chow，A. B。霍普和J. M.安德森（1989），Biochimica et BioproteicaActa，973，105-8]，在稳态闪光条件下和在背景远红光存在下，每次闪光从叶圆片中放出的氧给出了功能性光系统II（PS II）反应中心数目的有效测量。对这种直接和方便的方法进行了进一步的研究，以优化进行可靠测量的条件。 结果发现，为了获得较高的估计[PS II]，对应于几乎所有PS II反应中心，结合除草剂的功能，一种形式的“光激活”是必要的，经过长时间的黑暗预孵育。 没有足够数量的闪光后，长期黑暗的孵化，功能PS II反应中心的数量被低估。 提供光激活已经发生，所测量的功能PS II反应中心的数量是独立的闪光频率高达至少40 Hz。 结果强烈表明，在稳态，光限制光合作用，不存在任何实质性的部分非功能性或“慢”PS II反应中心。
It was shown briefly [W. S. Chow, A. B. Hope and J. M. Anderson (1989), Biochimica et Biophysica Acta, 973, 105-8] that the oxygen evolved per flash from leaf discs, under steady-state flashing conditions and in the presence of background far-red light, gave a valid measure of the number of functional photosystem II (PS II) reaction centres. Further work on this direct and convenient method has been done to optimise conditions for making reliable measurements. It is found that, to obtain the higher estimates of [PS II], corresponding to functionality of practically all PS II reaction centres that bind herbicides, a form of 'light activation' is necessary after a prolonged dark pre-incubation. Without a sufficient number of flashes being given following a long dark incubation, the number of functional PS II reaction centres was underestimated. Provided light activation had occurred, the measured number of functional PS II reaction centres was independent of flash frequencies up to at least 40 Hz. The results strongly suggest that, in steady-state, light-limited photosynthesis, there does not exist any substantial fraction of non-functional or 'slow' PS II reaction centres.