Mast Cells Are Identified in the Lung Parenchyma of Wild Mice, Which Can Be Recapitulated in Naturalized Laboratory Mice.
Mast Cells Are Identified in the Lung Parenchyma of Wild Mice, Which Can Be Recapitulated in Naturalized Laboratory Mice.
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DOI：
10.3389/fimmu.2021.736692
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发表时间：
2021
影响因子：
7.3
通讯作者：
Xiang Z
中科院分区：
文献类型：
--
作者：
Yeh YW;Chaudhuri AS;Zhou L;Fang Y;Boysen P;Xiang Z
有充分证据表明，与野生小鼠相比，在超卫生的无特定病原体 (SPF) 屏障中饲养和饲养的实验室小鼠的微生物群丰富度和复杂性有所降低。实验室小鼠严重缺乏肺实质肥大细胞。因此，我们的目的是研究自由生活的野生小鼠中肥大细胞的肺部分布。野生家鼠被困在挪威东南部和印度西孟加拉邦的亨塔巴德。 C57BL/6 实验室小鼠在特意建造的封闭环境中饲养，垫料取自自然环境，以使这些实验室小鼠的肠道微生物群与野生小鼠的肠道微生物群正常化，并在八周龄时收集后代进行研究。在挪威和印度野生小鼠的肺实质组织中，很容易识别出大量密度的肥大细胞，这与传统实验室 SPF 小鼠中肺实质肥大细胞的罕见分布形成鲜明对比。一致的是，野生小鼠肺部也表达了较高水平的干细胞因子，这是肥大细胞存活的关键生长因子。野生小鼠肺组织中的组胺含量较高。有趣的是，“归化”的 C57BL/6 实验室小鼠一生都生活在半自然环境中，其肺实质肥大细胞的密度相当高。我们的观察结果支持环境因素（可能通过调节微生物群）可能影响小鼠肺实质中肥大细胞的组织分布。
It is well documented that laboratory mice bred and maintained in ultra-hygienic specific pathogen-free (SPF) barriers display reduced richness and complexity of microbiota compared with wild mice. The laboratory mice profoundly lack lung parenchymal mast cells. Hence, we aimed to investigate the lung distribution of mast cells in free-living wild mice. Wild house mice were trapped in South-Eastern Norway and Hemtabad, West Bengal, India. C57BL/6 laboratory mice were bred in a purposefully built, closed environment with bedding material obtained from the natural environment in order to normalize the gut microbiota of these laboratory mice to that of the wild mice, and the offspring were collected for study at eight weeks of age. Mast cells were easily identified at a substantial density in the lung parenchymal tissues of wild mice from both Norway and India, which stands in clear contrast to the rare distribution of lung parenchymal mast cells in the conventional laboratory SPF mice. Consistently, wild mice also expressed higher pulmonary levels of stem cell factor, a critical growth factor for mast cell survival. Higher levels of histamine were recorded in the lung tissues of the wild mice. Interestingly, “naturalized” C57BL/6 laboratory mice which spent their entire life in a semi-natural environment developed lung parenchymal mast cells at an appreciable density. Our observations support that environmental factors, possibly through modulation of microbiota, may impact the tissue distribution of mast cells in mouse lung parenchyma.