The contractile vacuole network of Dictyostelium as a distinct organelle: its dynamics visualized by a GFP marker protein.
The contractile vacuole network of Dictyostelium as a distinct organelle: its dynamics visualized by a GFP marker protein.
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盘基网柄菌的收缩液泡网络作为一种独特的细胞器：其动态通过 GFP 标记蛋白可视化。
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
Günther Gerisch
中科院分区：
文献类型：
--
作者：
D. Gabriel;Ulrike Hacker;Jana Köhler;A. Müller;J. Schwartz;M. Westphal;Günther Gerisch
收缩液泡系统是由池和互连导管组成的渗透调节细胞器。大水池充当膀胱，周期性地与质膜融合，形成孔隙以排出水。为了在体内可视化整个网络并识别细胞组分中液泡复合物的成分，我们将一种特定的标记物引入盘基网柄菌细胞中，即 GFP 标记的 dajumin。该跨膜蛋白的 C 端 GFP 标记区域负责分类至收缩液泡复合体。 Dajumin-GFP 与质膜的结合可以忽略不计，表明其在膀胱排出过程中保留。荧光标记的细胞表面成分通过内吞作用被有效地内化，而没有观察到通过收缩液泡的显着循环。装载有酵母颗粒或液相标记的内体表明内吞途径与收缩液泡室的急剧分离。即使在有丝分裂期间收缩液泡系统分散后，dajumin-GFP 仍能区分囊泡和内体，并可视化细胞核周围网络的有丝分裂后重组。提出了高度辨别性的分选和膜融合机制来解释收缩液泡和内体区室的急剧分离。讨论了其他真核细胞中存在类似区室的证据。
The contractile vacuole system is an osmoregulatory organelle composed of cisternae and interconnecting ducts. Large cisternae act as bladders that periodically fuse with the plasma membrane, forming pores to expel water. To visualize the entire network in vivo and to identify constituents of the vacuolar complex in cell fractions, we introduced a specific marker into Dictyostelium cells, GFP-tagged dajumin. The C-terminal, GFP-tagged region of this transmembrane protein is responsible for sorting to the contractile vacuole complex. Dajumin-GFP negligibly associates with the plasma membrane, indicating its retention during discharge of the bladder. Fluorescent labeled cell-surface constituents are efficiently internalized by endocytosis, while no significant cycling through the contractile vacuole is observed. Endosomes loaded with yeast particles or a fluid-phase marker indicate sharp separation of the endocytic pathway from the contractile vacuole compartment. Even after dispersion of the contractile vacuole system during mitosis, dajumin-GFP distinguishes the vesicles from endosomes, and visualizes post-mitotic re-organization of the network around the nucleus. Highly discriminative sorting and membrane fusion mechanisms are proposed to account for the sharp separation of the contractile vacuole and endosomal compartments. Evidence for a similar compartment in other eukaryotic cells is discussed.
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液泡质子转位 ATP 酶参与盘基网柄菌内溶酶体系统和收缩液泡系统的多个步骤。
影响因子：
4
作者：
Temesvari,LA;Rodriguez-Paris,JM;Bush,JM;Zhang,L;Cardelli,JA
通讯作者：
Cardelli,JA
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nolta,KV;Padh,H;Steck,TL
通讯作者：
Steck,TL
酸性细胞内区室在盘基网柄菌溶酶体酶生物合成中的作用。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cardelli,JA;Richardson,J;Miears,D
通讯作者：
Miears,D
盘基网柄菌内吞液泡和酸体关联的重建。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Padh,H;Lavasa,M;Steck,TL
通讯作者：
Steck,TL
盘基网柄菌中的 Rab4 样 GTP 酶与收缩液泡复合体的网状膜和溶酶体中的 V-H( )-ATP 酶共定位。
影响因子：
4
作者：
Bush,J;Nolta,K;Rodriguez-Paris,J;Kaufmann,N;O'Halloran,T;Ruscetti,T;Temesvari,L;Steck,T;Cardelli,J
通讯作者：
Cardelli,J