Hexachromatic bioinspired camera for image-guided cancer surgery
Hexachromatic bioinspired camera for image-guided cancer surgery
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DOI：
10.1126/scitranslmed.aaw7067
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发表时间：
2021-05-05
影响因子：
17.1
通讯作者：
Gruev, Viktor
中科院分区：
文献类型：
--
作者：
Blair, Steven;Garcia, Missael;Gruev, Viktor
癌症影响着全世界三分之一的人。手术仍然是局部癌症的主要治疗选择，但良好的手术需要完全切除原发肿瘤并及时识别转移。为了扩大手术能力并提高患者的治疗效果，我们开发了一种六通道彩色/近红外图像传感器，其灵感来自螳螂虾视觉系统，该系统可在手术期间实现近红外荧光图像引导。螳螂虾独特的眼睛，它最大限度地提高了光子的数量和包含在其周围环境的每一个一瞥的信息量，是概括在我们的单芯片成像系统，集成了垂直堆叠的硅光电探测器和像素化光谱滤波器阵列。为了提供单个仪器无法提供的肿瘤位置信息，我们调整了三个颜色通道，以允许直观地观察手术过程，并调整了三个近红外通道，以允许突出显示癌组织的光学探头的多功能成像。在携带人类前列腺肿瘤的裸无胸腺小鼠中，我们的图像传感器能够同时检测两种肿瘤靶向荧光团，在估计92%的情况下区分患病组织和健康组织。它还允许提取近红外结构照明，使肿瘤和手术部位的三维地形图的映射误差在1.2 mm以内。在手术室中，在18名乳腺癌患者的手术切除过程中，我们的图像传感器使用临床批准的近红外荧光团进一步实现了前哨淋巴结标测。这种简单紧凑的架构所提供的灵活性和性能突出了生物传感器在图像引导手术中的优势。
Cancer affects one in three people worldwide. Surgery remains the primary curative option for localized cancers, but good prognoses require complete removal of primary tumors and timely recognition of metastases. To expand surgical capabilities and enhance patient outcomes, we developed a six-channel color/near-infrared image sensor inspired by the mantis shrimp visual system that enabled near-infrared fluorescence image guidance during surgery. The mantis shrimp's unique eye, which maximizes the number of photons contributing to and the amount of information contained in each glimpse of its surroundings, is recapitulated in our single-chip imaging system that integrates arrays of vertically stacked silicon photodetectors and pixelated spectral filters. To provide information about tumor location unavailable from a single instrument, we tuned three color channels to permit an intuitive perspective of the surgical procedure and three near-infrared channels to permit multifunctional imaging of optical probes highlighting cancerous tissue. In nude athymic mice bearing human prostate tumors, our image sensor enabled simultaneous detection of two tumor-targeted fluorophores, distinguishing diseased from healthy tissue in an estimated 92% of cases. It also permitted extraction of near-infrared structured illumination enabling the mapping of the three-dimensional topography of tumors and surgical sites to within 1.2-mm error. In the operating room, during surgical resection in 18 patients with breast cancer, our image sensor further enabled sentinel lymph node mapping using clinically approved near-infrared fluorophores. The flexibility and performance afforded by this simple and compact architecture highlights the benefits of biologically inspired sensors in image-guided surgery.