Spontaneous mucosal lymphocyte cytokine profiles in children with autism and gastrointestinal symptoms: Mucosal immune activation and reduced counter regulatory interleukin-10
Spontaneous mucosal lymphocyte cytokine profiles in children with autism and gastrointestinal symptoms: Mucosal immune activation and reduced counter regulatory interleukin-10
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DOI：
10.1007/s10875-004-6241-6
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发表时间：
2004-11-01
影响因子：
9.1
通讯作者：
Wakefield, AJ
中科院分区：
文献类型：
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作者：
Ashwood, P;Anthony, A;Wakefield, AJ
在一组患有自闭症谱系障碍（ASD）且有胃肠道（GI）症状的儿童中，已有淋巴细胞性小肠结肠炎的报道。本研究验证了一个假设，即这些自闭症儿童存在肠道黏膜免疫失调，促炎细胞因子产生增加。与有和没有黏膜炎症的发育正常儿童进行了比较。从21名自闭症儿童和65名发育正常的儿童对照者（其中38名有组织学炎症迹象）获取了十二指肠和结肠活检样本。通过多色流式细胞术检测自发细胞内肿瘤坏死因子α（TNFα）、白细胞介素 - 2（IL - 2）、白细胞介素 - 4（IL - 4）、干扰素γ（IFNγ）和白细胞介素 - 10（IL - 10）的CD3（+）淋巴细胞染色情况。与无炎症的对照组相比，自闭症儿童的十二指肠和结肠黏膜CD3（+）淋巴细胞计数升高（p
A lymphocytic enterocolitis has been reported in a cohort of children with autistic spectrum disorder (ASD) and gastrointestinal (GI) symptoms. This study tested the hypothesis that dysregulated intestinal mucosal immunity with enhanced proinflammatory cytokine production is present in these ASD children. Comparison was made with developmentally normal children with, and without, mucosal inflammation. Duodenal and colonic biopsies were obtained from 21 ASD children, and 65 developmentally normal paediatric controls, of which 38 had signs of histological inflammation. Detection of CD3(+) lymphocyte staining for spontaneous intracellular TNFalpha, IL-2, IL-4, IFNgamma, and IL-10, was performed by multicolor flow cytometry. Duodenal and colonic mucosal CD3(+) lymphocyte counts were elevated in ASD children compared with noninflamed controls (p