STRUCTURAL STUDIES OF THE O-POLYSACCHARIDE FROM THE LIPOPOLYSACCHARIDE OF MORAXELLA (BRANHAMELLA) CATARRHALIS SEROTYPE-A (STRAIN ATCC-25238)
STRUCTURAL STUDIES OF THE O-POLYSACCHARIDE FROM THE LIPOPOLYSACCHARIDE OF MORAXELLA (BRANHAMELLA) CATARRHALIS SEROTYPE-A (STRAIN ATCC-25238)
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DOI：
10.1016/0008-6215(94)80040-5
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发表时间：
1994-05-05
影响因子：
3.1
通讯作者：
WEINTRAUB, A
中科院分区：
文献类型：
--
作者：
EDEBRINK, P;JANSSON, PE;WEINTRAUB, A
采用温和酸解和凝胶渗透层析的方法制备A型卡他莫拉氏菌的脂多糖。通过甲基化分析、质谱学和核磁共振波谱确证了其结构。结果表明，O-抗原性多糖具有如下结构。对完整的脂多糖进行甲基化分析表明，脂类A部分由6-取代氨基葡萄糖残基组成。甲基化和甲醇裂解表明存在两个KDO残基，一个末端和一个4，5-取代残基。因此，终端KDo在4位置中替换分支点KDo。
The polysaccharide of the Moraxella (Branhamella) catarrhalis serotype A lipoporysaccharide was prepared by mild acid hydrolysis followed by gel permeation chromatography. The structure was established by methylation analysis, mass spectrometry, and NMR spectroscopy. It is concluded that the O-antigenic polysaccharide has the following structure.[GRAPHICS]Methylation analysis of the intact lipopolysaccharide showed that the lipid A portion consisted of 6-substituted glucosamine residues. Methylation followed by methanolysis showed that two Kdo residues were present, one terminal and one 4,5-substituted residue. A terminal Kdo thus substitutes the branch-point Kdo in the 4-position.