A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development
A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development
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DOI：
10.1053/gast.2000.19576
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发表时间：
2000-11-01
期刊：
影响因子：
29.4
通讯作者：
Pasricha, PJ
中科院分区：
文献类型：
--
作者：
Al-Chaer, ED;Kawasaki, M;Pasricha, PJ
背景与目的：肠易激综合征(IBS)是一种以腹痛为特征的常见疾病，其特征是内脏感觉刺激的感觉改变。这种所谓的内脏痛觉过敏发生在周围器官没有可检测到的器质性疾病的情况下，可能会导致正常或生理性的宫缩被认为是疼痛的。尽管IBS的发病机制仍然是推测的，可能是多因素的，但一个流行的范式是，尽管启动事件完全解决，但短暂的伤害性事件会导致神经痛觉回路的长期敏感化。方法：新生雄性SD大鼠在出生后第8天至第21天接受机械或化学结肠刺激，成年后进行测试。记录大鼠结肠扩张时的腹部撤退反射和内脏敏感神经元的反应。结果：对新生儿结肠的刺激可导致慢性内脏高敏感性，具有痛觉过敏和痛觉过敏的特征，并伴有中枢神经敏化，但无明显的外周病理改变。结论：这些结果在很大程度上与IBS患者的观察结果一致，为研究IBS提供了一个新的动物模型，并验证了功能性腹痛的神经源性成分，鼓励了健康洞穴和研究的新方法。
Background & Aims: The irritable bower syndrome (IBS) is a common disorder characterized by abdominal pain in the setting of altered perception of viscerosensory stimuli. This so-called visceral hyperalgesia occurs in the absence of detectable organic disease in the peripheral organs and may cause normal or physiologic contractions to be perceived as painful. Although the pathogenesis of IBS remains speculative and is probably multifactorial, a prevailing paradigm is that transient noxious events lead to long-lasting sensitization of the neural pain circuit, despite complete resolution of the initiating event. Methods: Neonatal male Sprague-Dawley rats received either mechanical or chemical colonic irritation between postnatal days 8 and 21 and were tested when they became adults. The abdominal withdrawal reflex and the responses of viscerosensitive neurons were recorded during colon distention. Results: Colon irritation in neonates, but not in adults, results in chronic visceral hypersensitivity, with characteristics of allodynia and hyperalgesia, associated with central neuronal sensitization in the absence of identifiable peripheral pathology. Conclusions: These results concur largely with observations in patients with IBS, providing a new animal model to study IBS and validating a neurogenic component of functional abdominal pain that encourages novel approaches to health cave and research.