Granulocyte chemotaxis: an improved in vitro assay employing 51 Cr-labeled granulocytes.
Granulocyte chemotaxis: an improved in vitro assay employing 51 Cr-labeled granulocytes.
复制标题
粒细胞趋化性：采用 51 Cr 标记粒细胞的改进体外测定。
作者：
J. Gallin;R. A. Clark;H. Kimball
本文介绍了一种用铬-51标记细胞和双滤膜系统测定粒细胞趋化性的放射性分析法。与传统的Boyden室技术相比，该方法具有显著的优点，包括：a）消除了繁琐的过滤染色和显微镜计数，从而大大节省了实验室时间，B）消除了观察者的主观性，c）在宽范围的细胞反应上具有优异的灵敏度，而不增加变异性，d）监测趋化性室的所有隔室中的细胞事件的能力，和e）用于在不同实验室中标准化嗜中性粒细胞趋化性的手段。
A radioassay of granulocyte chemotaxis employing chromium-51 labeled cells and a double micropore filter system is presented. In comparison to the conventional Boyden chamber technique, this method offers significant advantages including: a) elimination of tedious filter staining and microscope counting resulting in a considerable saving in laboratory time, b) elimination of observer subjectivity, c) excellent sensitivity over a wide range of cell responses with no increase in variability, d) ability to monitor cellular events in all compartments of the chemotaxis chamber and e) a means for standardization of neutrophil chemotaxis among different laboratories.