Amino acid sequence of band-3 protein from rainbow trout erythrocytes derived from cDNA.
Amino acid sequence of band-3 protein from rainbow trout erythrocytes derived from cDNA.
复制标题
来自虹鳟鱼红细胞的 band-3 蛋白的氨基酸序列，源自 cDNA。
作者：
S. Hübner;F. Michel;V. Rudloff;H. Appelhans
在这份报告中，我们提出了第一个完整的带-3（band-3）cDNA序列的变温低等脊椎动物。通过对虹鳟鱼红细胞阴离子交换蛋白带3（AE 1）cDNA克隆的核苷酸序列测定，确定了AE 1的一级结构。重叠克隆的总长度为3827 bp，其中5 '端非翻译区为150 bp，开放阅读框为2754 bp，3'端非翻译区为924 bp。鳟鱼红细胞带3蛋白由918个氨基酸组成，分子量为101827 Da，与已发表的带3蛋白跨膜序列同源性为60-65%。在鳟鱼带-3蛋白的膜相关结构域中额外插入了24个氨基酸残基，直到现在为止，这被认为是哺乳动物带-3相关蛋白的一般特征。
In this report we present the first complete band-3 cDNA sequence of a poikilothermic lower vertebrate. The primary structure of the anion-exchange protein band 3 (AE1) from rainbow trout erythrocytes was determined by nucleotide sequencing of cDNA clones. The overlapping clones have a total length of 3827 bp with a 5'-terminal untranslated region of 150 bp, a 2754 bp open reading frame and a 3'-untranslated region of 924 bp. Band-3 protein from trout erythrocytes consists of 918 amino acid residues with a calculated molecular mass of 101 827 Da. Comparison of its amino acid sequence revealed a 60-65% identity within the transmembrane spanning sequence of band-3 proteins published so far. An additional insertion of 24 amino acid residues within the membrane-associated domain of trout band-3 protein was identified, which until now was thought to be a general feature only of mammalian band-3-related proteins.