3D Porous Chitosan-Alginate Scaffolds Promote Proliferation and Enrichment of Cancer Stem-Like Cells.
3D Porous Chitosan-Alginate Scaffolds Promote Proliferation and Enrichment of Cancer Stem-Like Cells.
复制标题
3D多孔壳聚糖 - 甲酸盐支架促进癌症干细胞的增殖和富集。
DOI：
10.1039/c6tb01713d
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发表时间：
2016-10-14
期刊：
影响因子：
--
通讯作者：
Zhang M
中科院分区：
文献类型：
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作者：
Florczyk SJ;Kievit FM;Wang K;Erickson AE;Ellenbogen RG;Zhang M
癌症干细胞正日益成为新癌症治疗开发的主要目标。在体外研究它们的瞬时行为的能力将为更有效的疗法的高通量测试提供机会。我们先前已经证明了使用3D多孔壳聚糖-藻酸盐（CA）支架来促进恶性胶质母细胞瘤中的癌症干细胞样细胞（CSC）增殖和富集。在这里，我们使用3D多孔CA支架，以促进癌症干细胞样细胞富集在前列腺癌，肝癌和乳腺癌的细胞系，并研究CA支架中培养的细胞的增殖，形态和基因表达相比，2D控制。与单层相反，所有三种癌症类型的3D CA支架培养物均显示增殖减少、肿瘤球状体形成以及CSC相关标记基因（CD133和NANOG）表达增加。此外，我们提出了一个推定的机制，癌症干细胞样细胞富集CA支架。这项研究表明，CA支架中的癌症干细胞样细胞富集是一个强大的过程，不限于特定的癌症类型。
Cancer stem cells are increasingly becoming a primary target for new cancer treatment development. The ability to study their transient behavior in vitro will provide the opportunity for high-throughput testing of more effective therapies. We have previously demonstrated the use of 3D porous chitosan-alginate (CA) scaffolds to promote cancer stem-like cell (CSC) proliferation and enrichment in glioblastoma. Here we use 3D porous CA scaffolds to promote cancer stem-like cell enrichment in cell lines from prostate, liver, and breast cancers, and investigate the proliferation, morphology, and gene expressions of cells cultured in CA scaffolds as compared to 2D controls. The 3D CA scaffold cultures for all three cancer types showed reduced proliferation, formation of tumor spheroids, and increased expression of CSC associated mark genes (CD133 and NANOG), as opposed to monolayers. Additionally, we present a putative mechanism for the cancer stem-like cell enrichment on CA scaffolds. This study demonstrates that the cancer stem-like cell enrichment in CA scaffolds is a robust process that is not restricted to particular cancer types.