Synthesis of a 3-methyluridine phosphoramidite to investigate the role of methylation in a ribosomal RNA hairpin.
Synthesis of a 3-methyluridine phosphoramidite to investigate the role of methylation in a ribosomal RNA hairpin.
复制标题
合成 3-甲基尿苷亚磷酰胺，研究甲基化在核糖体 RNA 发夹中的作用。
DOI：
10.1016/s0968-0896(01)00283-8
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发表时间：
2002
影响因子：
3.5
通讯作者：
Chow,ChristineS
中科院分区：
文献类型：
--
作者：
Chui,HelenMP;Meroueh,May;Scaringe,StephenA;Chow,ChristineS
The synthesis of a 5′-O-BzH-2′-O-ACE-protected-3-methyluridine phosphoramidite is reported [BzH, benzhydryloxy-bis(trimethylsilyloxy)silyl; ACE, bis(2-acetoxyethoxy)methyl]. The phosphoramidite was employed in solid-phase RNA synthesis to generate a series of RNA hairpins containing single or multiple modifications, including the common nucleoside pseudouridine. Three 19-nucleotide hairpin RNAs that represent the 1920-loop region (G1906–C1924) of Escherichia coli 23S ribosomal RNA were generated. Modifications were present at positions 1911, 1915, and 1917. The stabilities and structures of the three RNAs were examined by using thermal melting, circular dichroism, and NMR spectroscopy
