Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol
Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol
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DOI：
10.1016/j.biortech.2010.03.143
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发表时间：
2010-09-01
影响因子：
11.4
通讯作者：
Li, Sha
中科院分区：
文献类型：
--
作者：
Gao, Jian;Xu, Hong;Li, Sha
开发了多粘类芽孢杆菌ZJ-9一步发酵菊芋块茎粗菊粉提取物生产R,R-2,3-丁二醇(R,R-2,3-BD)的培养基。根据Plackett-Burman实验设计的结果，发现菊粉、K2HPO4和NH4Cl是发酵的关键因素。通过最陡上升路径考察了三个因素的最佳浓度范围，根据Box-Behnken设计进一步考察其最佳浓度，分别确定为77.14 g/L、3.09 g/L和0.93 g/L。在最佳条件下，所得R,R-2,3-BD的浓度为36·92 g/L，光学纯度超过98%。与其他研究的碳资源相比，菊粉粗提取物的发酵获得了最高的R,R-2,3-BD产率。该工艺采用菊粉一步发酵，无需进一步水解，大大降低了原料成本，有利于其实际应用。 (C) 2010 Elsevier Ltd. 版权所有
The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed Inulin, K2HPO4 and NH4Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.14 g/L, 3.09 g/L and 0.93 g/L, respectively Under the optimal conditions, the concentration of the obtained R,R-2,3-BD was 36 92 g/L, at more than 98% optical purity Compared with other investigated carbon resources, fermentation of the raw inulin extract afforded the highest yield of R,R-2,3-BD This process featured one-step fermentation of inulin without further hydrolyzing, which greatly decreased the raw material cost and thus facilitated its practical application. (C) 2010 Elsevier Ltd. All rights reserved