Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells
Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells
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DOI：
10.1016/j.bioelechem.2006.05.008
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发表时间：
2007-05-01
影响因子：
5
通讯作者：
Lee, Yi-Kuen
中科院分区：
文献类型：
--
作者：
He, Huiqi;Chang, Donald C.;Lee, Yi-Kuen
本研究利用MEMS技术制备了一种三维（31）电极的微电穿孔（EP）细胞芯片，并在宫颈癌（HeLa）细胞上进行了实验。通过对阈值电场和脉冲持续时间的大量统计数据进行分析，构建了一个电位“相图”，该相图描绘了1)五种不同大小分子的有效电位和2)单细胞水平的电解边界。此外，这些边界曲线（即电场与脉冲持续时间）成功地与具有三个常数的指数函数拟合。我们发现，当分子尺寸增大时，相应的电穿孔边界更接近于电池的电解边界。基于对5种不同大小分子的2000多个单细胞测量，发现分子的临界大小约为40kDa。与传统仪器相比，基于mems的微电穿孔芯片可以大大缩短实验时间。(C) 2006 Elsevier B.V.版权所有
In this study, a new micro electroporation (EP) cell chip with three-dimensional (31)) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP "phase diagram", which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time. (C) 2006 Elsevier B.V. All rights reserved.