The role of detoxifying enzymes in the resistance of the cowpea aphid (Aphis craccivora Koch) to thiamethoxam
The role of detoxifying enzymes in the resistance of the cowpea aphid (Aphis craccivora Koch) to thiamethoxam
复制标题
DOI：
10.1515/jppr-2016-0010
复制
发表时间：
2016-01-01
影响因子：
1.1
通讯作者：
Kandil, Mohammed Abd El-Hady
中科院分区：
文献类型：
--
作者：
Abdallah, Ibrahim Saleh;Abou-Yousef, Hala Mohamed;Kandil, Mohammed Abd El-Hady
豇豆蚜（Aphis craccivora Koch）是一种危害多种作物的严重害虫。我们进行了生化研究，以阐明代谢酶的作用，赋予抗噻虫嗪，在两个菌株（抗性和敏感）的豇豆蚜虫。生测结果表明，噻虫嗪筛选菌株经12代的连续筛选，抗性提高了48倍。这种抗性菌株还表现出对所测试的氨基甲酸酯、抗蚜威和丁硫克百威、有机磷（马拉硫磷、杀螟松和甲基毒死蜱）和类烟碱（啶虫脒）的交叉抗性。增效作用研究表明，S，S，S-三丁基三硫代磷酸酯（DEF），一种已知的酯酶抑制剂，增加噻虫嗪在抗性菌株的毒性比敏感菌株的5.58倍。此外，生化测定表明，羧酸酯酶活性在抗性品系中比在敏感品系中高30倍。此外，谷胱甘肽S-转移酶（GST）和多功能氧化酶（mfo）的酶活性仅在抗性菌株中分别增加了3.7和2.7倍，与敏感（对照）。一般来说，我们的研究结果表明，较高的活性的解毒酶，特别是羧酸酯酶，在豇豆蚜虫的抗性菌株，显然有一个显着的作用，赋予抗性噻虫嗪，虽然其他机制可能作出贡献。
The cowpea aphid (Aphis craccivora Koch) is considered a serious insect pest attacking several crops. We carried out biochemical studies to elucidate the role of the metabolising enzymes in conferring resistance to thiamethoxam, in two strains (resistant and susceptible) of the cowpea aphid. Bioassay experiments showed that the thiamethoxam selected strain developed a 48 fold resistance after consecutive selection with thiamethoxam for 12 generations. This resistant strain also exhibited cross-resistance to the tested carbamates; pirimicarb and carbosulfan, organophosphorus (malathion, fenitrothion, and chlorpyrifos-methyl), and the neonicotinoid (acetamiprid). Synergism studies have indicated that S, S, S-tributyl phosphorotrithioate (DEF), a known inhibitor for esterases, increased thiamethoxam toxicity 5.58 times in the resistant strain compared with the susceptible strain. Moreover, the biochemical determination revealed that carboxylestersae activity was 30 times greater in the resistant strain than in the susceptible strain. In addition, the enzyme activity of glutathione S-transferase (GST) and mixed function oxidases (mfo) increased only in the resistant strain 3.7 and 2.7 times, respectively, in relation to the susceptible (the control). Generally, our results suggest that the higher activity of the detoxifying enzymes, particularly carboxylesterase, in the resistant strain of the cowpea aphid, apparently have a significant role in endowing resistance to thiamethoxam, although additional mechanisms may contribute.