COMPUTED CIRCULAR DICHROISM SPECTRA FOR EVALUATION OF PROTEIN CONFORMATION
COMPUTED CIRCULAR DICHROISM SPECTRA FOR EVALUATION OF PROTEIN CONFORMATION
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DOI：
10.1021/bi00838a031
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发表时间：
1969-01-01
期刊：
影响因子：
2.9
通讯作者：
FASMAN, GD
中科院分区：
文献类型：
--
作者：
GREENFIE.N;FASMAN, GD
已在 190-250-μ 区域计算了包含不同数量的螺旋、/3-折叠片和随机线圈段的聚-L-赖氨酸的圆二色性曲线。讨论了这些曲线在确定蛋白质构象中的应用。几种蛋白质的圆二色性曲线，其三维结构是从 X 射线衍射研究中已知的，已通过 208-240-μ 区域中三个参考结构的线性组合进行拟合。结果表明，这些计算
Circular dichroism curves of poly-L-lysine con-taining varying amounts of a helix,/3-pleatedsheet, and random coil segments have been computed in the 190-250-µ region. The application of these curves for determining protein conformation is discussed. The circular dichroism curves of several proteins, whose three-dimensional structures are known from X-ray diffraction studies, have been fitted by a linear combination of the threereference structures in the 208-240-µ region. Theresults show that these computed