Myogenic activation of canine small renal arteries after nonchemical removal of the endothelium.
Myogenic activation of canine small renal arteries after nonchemical removal of the endothelium.
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非化学去除内皮后犬小肾动脉的肌源性激活。
DOI：
10.1152/ajpheart.1994.267.1.h302
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Lombard,JH
中科院分区：
文献类型：
--
作者：
Liu,Y;Harder,DR;Lombard,JH
通过测量在通过机械摩擦或空气灌注去除内皮之前和之后跨壁压逐步升高期间的血管直径，评价犬肾的分离的小动脉（200-500微米）的肌原性活化。生肌激活不受机械摩擦的影响，但空气灌注导致动脉收缩并减弱生肌反应;即，血管直径随着跨壁压的升高而增加。然而，空气灌注的动脉仍然对去甲肾上腺素产生反应而收缩，并且在用无Ca（2+）溶液灌注期间扩张，这表明肌源性反应的丧失不是由于血管不能收缩所致。用1 μ M去甲肾上腺素收缩的完整动脉表现出正常的肌源性反应，表明空气灌注血管中肌源性激活的丧失不是继发于动脉收缩的非特异性效应。这些结果表明，小肾动脉的肌源性激活是独立的内皮细胞和内皮细胞去除的方法可能会影响的结论，内皮细胞介导的肌源性反应的作用。
Myogenic activation of isolated small arteries (200-500 microns) of the dog kidney was evaluated by measuring vessel diameters during stepwise elevations of transmural pressure before and after removal of the endothelium by mechanical rubbing or air perfusion. Myogenic activation was unaffected by mechanical rubbing, but air perfusion caused the arteries to constrict and attenuated the myogenic response; i.e., vessel diameters increased as transmural pressure was elevated. However, air-perfused arteries still constricted in response to norepinephrine and dilated during superfusion with Ca(2+)-free solution, demonstrating that loss of the myogenic response did not result from an inability of the vessel to contract. Intact arteries contracted with 1 microM norepinephrine exhibited normal myogenic responses, demonstrating that loss of myogenic activation in the air-perfused vessels was not a nonspecific effect secondary to constriction of the artery. These results suggest that myogenic activation of small renal arteries is independent of the endothelium and that the method of endothelial removal may affect conclusions regarding the role of the endothelium in mediating the myogenic response.