Expression of serum- and glucocorticoid-inducible kinase is regulated in an experience-dependent manner and can cause dendrite growth
Expression of serum- and glucocorticoid-inducible kinase is regulated in an experience-dependent manner and can cause dendrite growth
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DOI：
10.1523/jneurosci.0006-05.2005
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发表时间：
2005-07-27
影响因子：
5.3
通讯作者：
Kalb, RG
中科院分区：
文献类型：
--
作者：
David, S;Stegenga, SL;Kalb, RG
动物在出生后早期的关键时期与其环境的相互作用对感觉和运动系统的结构和功能具有终生影响。为了深入了解经验依赖型发展的分子机制，我们要求幼鼠适应新的环境，从而产生新的运动行为。在地球重力场（1G）中出生的老鼠随后在无重力（微重力）或1G条件下饲养2周。使用微阵列对基因表达进行比较，鉴定出一组差异调节的转录本。我们在此报告，与 1G 饲养的对照相比，微重力饲养的动物脊髓组织中血清和糖皮质激素诱导激酶 (SGK) 的丰度有所增加。 SGK表达的诱导也可以通过在体外向动物或神经元施用1G糖皮质激素来实现。神经元中组成型活性 SGK 的表达导致神经元树突及其分支的形成。糖皮质激素也会导致树突细化，这种作用可以通过抑制 SGK 活性来消除。 SGK 表达水平的变化可能是成熟神经元特性的经验依赖性习得机制的一部分。
The interaction of an animal with its environment during a critical period in early postnatal life has lifelong effects on the structure and function of sensory and motor systems. To gain insight into the molecular mechanisms of experience- dependent development, we challenged young rats to adapt to a new environment that engenders novel motor behavior. Rats born in the gravitational field ( 1G) of the earth subsequently were reared for 2 weeks either in the absence of gravity ( microgravity) or at 1G. A comparison of gene expression using microarrays led to the identification of a panel of differentially regulated transcripts. We report here that the abundance of serum- and glucocorticoid- inducible kinase ( SGK) is increased in spinal cord tissue from animals reared in microgravity in comparison with 1G-reared controls. The induction of SGK expression also can be achieved by administration of glucocorticoids to animals at 1G or neurons in vitro. Expression of constitutively active SGK in neurons leads to the elaboration of neuronal dendrites and their branching. Glucocorticoids also lead to dendrite elaboration, and this effect can be abrogated by inhibiting SGK activity. Changes in the level of expression of SGK could be part of the mechanism for experience- dependent acquisition of mature neuronal properties.