Arachidonic acid induced degranulation of rabbit peritoneal neutrophils.
Arachidonic acid induced degranulation of rabbit peritoneal neutrophils.
复制标题
花生四烯酸诱导兔腹膜中性粒细胞脱粒。
DOI：
10.1016/0006-291x(79)91678-4
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发表时间：
1979
影响因子：
3.1
通讯作者：
R. Sha’afi
中科院分区：
文献类型：
--
作者：
P. Naccache;H. Showell;Elmer L. Becker;R. Sha’afi
我们发现花生四烯酸快速、选择性地诱导细胞松弛素 B 处理的兔腹膜中性粒细胞释放溶酶体酶。 5, 8, 11, 14-二十碳四烯酸抑制花生四烯酸诱导的释放，表观 KD 为 1.5 × 10−6M。 5,8,11,14-二十碳四烯酸 (2.5 × 10−5M) 也会抑制趋化因子和 A23187 在细胞松弛素 B 存在下诱导的释放，但不影响单独由 A23187 诱导的脱颗粒。这些观察结果强烈表明花生四烯酸代谢物在兔中性粒细胞生理学中的作用。
We have found that arachidonic acid rapidly and selectively induces the release of lysosomal enzymes from cytochalasin B treated rabbit peritoneal neutrophils. 5, 8, 11, 14-eicosatetraynoic acid inhibits the arachidonate induced release with an apparent KDof 1.5 × 10−6M. 5,8,11,14-eicosatetraynoic acid (2.5 × 10−5M also inhibits the chemotactic factors and the A23187 induced release in the presence of cytochalasin B but does not affect the degranulation induced by A23187 alone. These observations strongly suggest a role for arachidonate metabolites in rabbit neutrophil physiology.