Transient receptor potential vanilloid subfamily 1 expressed in pancreatic islet β cells modulates insulin secretion in rats
Transient receptor potential vanilloid subfamily 1 expressed in pancreatic islet β cells modulates insulin secretion in rats
复制标题
DOI：
10.1016/j.bbrc.2004.06.149
复制
发表时间：
2004-08-11
影响因子：
3.1
通讯作者：
Hibi, T
中科院分区：
文献类型：
--
作者：
Akiba, Y;Kato, S;Hibi, T
辣椒素敏感传入神经元包括瞬时受体电位香草酸亚家族1、TRPV 1和神经激素肽参与胰腺内分泌的生理调节。然而，辣椒素对胰岛素分泌的直接影响仍然未知。我们目前的研究表明，TRPV 1在大鼠胰腺的胰岛β细胞以及神经元中表达，并且还在大鼠β细胞系中表达。RIN和INS 1。辣椒素（10（-11）-10（-9）M）剂量依赖性地增加RIN细胞的胰岛素分泌，并且该作用被TRPV 1抑制剂辣椒平或EDTA抑制。全身性辣椒素（10 mg/kg，s.c.）治疗后1 h血浆胰岛素水平升高。我们首次证明了TRPV 1在大鼠胰岛细胞中功能性表达，并作为钙通道在胰岛素分泌中发挥作用。本研究为辣椒素对摄食和能量消耗的影响以及对胰腺内分泌的病理生理调节提供了理论依据。(C)2004年爱思唯尔公司All rights reserved.
Capsaicin-sensitive afferent neurons including transient receptor potential vanilloid subfamily 1, TRPV1, and neurohormonal peptides participate in the physiological regulation of pancreatic endocrine. However, the direct effect of capsaicin on insulin secretion remains unknown. Our present Study showed that TRPV1 is expressed in islet beta cells as well as in neurons in rat pancreas, and also in rat beta cell lines. RIN and INS1. Capsaicin (10(-11)-10(-9) M) dose-dependently increased insulin secretion from RIN cells, and this effect was inhibited by either a TRPV1 inhibitor capsazepine or EDTA. Systemic capsaicin (10 mg/kg, s.c.) increased plasma insulin level 1 h after the treatment. We demonstrated for the first time that TRPV1 is functionally expressed in rat islet cells and plays a role in insulin secretion as a calcium channel. This study may account for the influences of capsaicin on the food intake and energy consumption as well as on the pathophysiological regulation of pancreatic endocrine. (C) 2004 Elsevier Inc. All rights reserved.