Free nitrous acid inhibition on the aerobic metabolism of poly-phosphate accumulating organisms
Free nitrous acid inhibition on the aerobic metabolism of poly-phosphate accumulating organisms
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DOI：
10.1016/j.watres.2010.07.075
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发表时间：
2010-12-01
期刊：
影响因子：
12.8
通讯作者：
Yuan, Zhiguo
中科院分区：
文献类型：
--
作者：
Pijuan, Maite;Ye, Liu;Yuan, Zhiguo
在全规模污水处理系统中，除磷通常与除氮同时进行。亚硝酸盐是硝化和反硝化过程的中间产物，可以在反应器中积累。研究了亚硝酸盐/游离亚硝酸(FNA)对聚磷菌(PAO)好氧代谢的抑制作用。采用高浓缩度(90+/-5%)的磷酸盐假丝酵母培养物，在不同的亚硝酸盐和pH水平下进行了一系列的间歇实验。FNA被发现抑制了PAO执行的所有关键的有氧代谢过程，即PHA氧化、磷吸收、糖原补充和生长。对合成代谢过程(生长、磷吸收和糖原产生)的抑制作用远强于对分解代谢过程(PHA氧化)的抑制作用。当FNA浓度约为0.5×10~(-3)mg HNO2-N/L(相当于2.0mgHNO2--N/L)时，对所有合成代谢过程均有50%的抑制作用，而当FNA浓度约为6.0×10~(-3)mg HNO2-N时，则出现完全抑制。这些浓度可以在通过亚硝酸盐途径实现脱氮的全规模废水处理系统中找到。相比之下，在FNA浓度为2.0×10~(-3)-10.0×10~(-3)mg HNO2-N/L的范围内，PHA的氧化速率保持在最高速率的40%~50%。有趣的是，在好氧条件下(DO约为3 mg/L)，PAO能够还原亚硝酸盐，其速率随着FNA浓度的增加而显著增加。对磷吸收的抑制是可逆的。(C)2010爱思唯尔有限公司。保留所有权利。
In full-scale wastewater treatment systems, phosphorus removal typically occurs together with nitrogen removal. Nitrite, an intermediate of both the nitrification and denitrification processes, can accumulate in the reactor. The inhibitory effect of nitrite/free nitrous acid (FNA) on the aerobic metabolism of poly-phosphate accumulating organisms (PAOs) is investigated. A culture highly enriched (90 +/- 5%) in Candidatus "Accummulibacter phosphatis", a well-known PAO, was used to perform a series of batch experiments at various nitrite and pH levels. FNA was found to inhibit all key aerobic metabolic processes performed by PAOs, namely PHA oxidation, phosphate uptake, glycogen replenishment and growth. The inhibitory effect on the anabolic processes (growth, phosphate uptake and glycogen production) was much stronger than that on the catabolic processes (PHA oxidation). 50% inhibition on all anabolic processes occurred at FNA concentrations of approximately 0.5 x 10(-3) mg HNO2-N/L (equivalent to 2.0 mg NO2--N/L at pH 7.0), while full inhibition occurred at FNA concentrations of approximately 6.0 x 10(-3) mg HNO2-N. These concentrations could be found in full-scale wastewater treatment systems that achieve nitrogen removal via the nitrite pathway. In comparison, PHA oxidation remained at 40-50% of the highest rate at FNA concentrations in the range 2.0 x 10(-3)-10.0 x 10(-3) mg HNO2-N/L. Interestingly, PAOs were able to reduce nitrite under aerobic conditions (DO approximate to 3 mg/L), with the rate increasing substantially with the FNA concentration. The inhibition on phosphate uptake was found to be reversible. (C) 2010 Elsevier Ltd. All rights reserved.